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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- R34677 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Doublecortin Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific Doublecortin antibody is suitable for use in Western blot/Immunohistochemistry/ELISA applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the Doublecortin antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse fetal brain lysate with Doublecortin antibody at 0.01ug/ml. Predicted molecular weight: 40-50 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC testing of human HepG2 cells with Alexa Fluor 488 secondary (1ug/ml) and Doublecortin antibody (green, 5ug/ml). Blue = DAPI nuclear counterstain.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human cerebellum tissue with (top) and without (bottom) Doublecortin antibody at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
