PA3-006

antibody from Invitrogen Antibodies

Targeting: GPR18

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA3-006

Provider

Invitrogen Antibodies

Product name

GPR18 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

IHC (P) analysis shows positive staining of GPR18 in human enteric ganglion tissue. WB analysis shows GPR18 in glycoprotein-enriched fractions from GPR18 overexpressing 293 cells. A band of similar MW was also detected in GPR3 overexpressing 293 cells, suggesting some cross-reactivity with GPR3 or enhanced detection of basal GPR18 in the enriched fractions. While GPR18 has a theoretical MW of ~ 38kD, the observed band in a WB runs at ~70kD due to enrichment of the glycosylated receptor.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

-20°C

References

Submitted references

Resolvin D2 uses multiple Ca(2+) -dependent signaling pathways to stimulate mucin secretion in rat and human conjunctival goblet cells.

Botten N, Hodges RR, Bair J, Utheim TP, Serhan CN, Yang M, Dartt DA
Journal of cellular physiology 2022 Oct;237(10):3816-3833

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GPR18 was performed on human enteric ganglion tissue. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with a GPR18 polyclonal antibody (Product # PA3-006) at a dilution of 1:3000, overnight at 4C in a humidified chamber. Tissues were washed, and detection was performed using an ABC kit composed of biotinylated goat anti-rabbit IgG, peroxidase-conjugated avidin, and 3-amino-9-ethylcarbazole (AEC) substrate in acetate buffer. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting.