BS3462

antibody from Bioworld Technology, Inc

Targeting: NPHS1 CNF, NPHN

Immunohistochemistry

Antibody data

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Validation data

Product number: BS3462 - Provider product page
Provider: Bioworld Technology, Inc
Proper citation: Bioworld Technology Cat#BS3462, RRID:AB_1661928
Product name: Nephrin (H892) polyclonal antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide, corresponding to amino acids 861-910 of Human Nephrin.
Description: Nephrin is a member of the immunoglobulin family of cell adhesion molecules that localizes to opposing sites of the secondary foot processes formed by podocytes, a specialized epithelial cell that ensures size- and charge-selective ultrafiltration. The human nephrin gene maps to chromosome 19q13.1 and encodes a 1,241 amino acid protein that contains a transmembrane domain, 8 Ig-like modules, and 1 fibronectin III-like module. Nephrin is expressed in embryonic and adult kidneys and localizes to glomerular podocytes and the glomerular slit diaphragm. Nephrin stimulates mitogen-activated protein kinases and is enhanced by podocin, which binds to the cytoplasmic tail of nephrin.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100ul
Concentration: 1 mg/ml
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

NPHS1 protein structure - BS3462 shown in red.

Supportive validation

Submitted by: Bioworld Technology, Inc (provider)
Main image
Experimental details: Western blot (WB) analysis of Nephrin (H892) pAb at 1:1000 dilution Lane1:The muscle tissue lysate of Rat(47ug) Lane2:HEK293T whole cell lysate(41ug) Lane3:Jurkat whole cell lysate(41ug) Lane4:H9C2 whole cell lysate(60ug)