Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A01991 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Nephrin/NPHS1 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Nephrin(NPHS1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Exosomal miR-223-3p from bone marrow mesenchymal stem cells targets HDAC2 to downregulate STAT3 phosphorylation to alleviate HBx-induced ferroptosis in podocytes.
MicroRNA-223 downregulation promotes HBx-induced podocyte pyroptosis by targeting the NLRP3 inflammasome.
Serum and Glucocorticoid-Inducible Kinase 3/Nedd4-2 Signaling Pathway Participates in Podocyte Injury by Regulating the Stability of Nephrin.
Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation.
Human anti-thrombospondin type 1 domain-containing 7A antibodies induce membranous nephropathy through activation of lectin complement pathway.
Chen Y, Yang X, Feng M, Yu Y, Hu Y, Jiang W
Frontiers in pharmacology 2024;15:1327149
Frontiers in pharmacology 2024;15:1327149
MicroRNA-223 downregulation promotes HBx-induced podocyte pyroptosis by targeting the NLRP3 inflammasome.
Yu Y, Dong H, Zhang Y, Sun J, Li B, Chen Y, Feng M, Yang X, Gao S, Jiang W
Archives of virology 2022 Sep;167(9):1841-1854
Archives of virology 2022 Sep;167(9):1841-1854
Serum and Glucocorticoid-Inducible Kinase 3/Nedd4-2 Signaling Pathway Participates in Podocyte Injury by Regulating the Stability of Nephrin.
Dong QQ, Li ZF, Zhang H, Shu HP, Tu YC, Liao QQ, Yao LJ
Frontiers in physiology 2021;12:810473
Frontiers in physiology 2021;12:810473
Protective Effects of Thalidomide on High-Glucose-Induced Podocyte Injury through In Vitro Modulation of Macrophage M1/M2 Differentiation.
Liao H, Li Y, Zhang X, Zhao X, Zheng D, Shen D, Li R
Journal of immunology research 2020;2020:8263598
Journal of immunology research 2020;2020:8263598
Human anti-thrombospondin type 1 domain-containing 7A antibodies induce membranous nephropathy through activation of lectin complement pathway.
Wang Z, Wen L, Dou Y, Zhao Z
Bioscience reports 2018 Jun 29;38(3)
Bioscience reports 2018 Jun 29;38(3)
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Nephrin using anti-Nephrin antibody (A01991). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat kidney tissue lysates, lane 2: mouse kidney tissue lysates, lane 3: 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nephrin antigen affinity purified polyclonal antibody (Catalog # A01991) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nephrin at approximately 134KD. The expected band size for Nephrin is at 134KD.
- Additional image