LS-C747566

NPHS1 antibody from LifeSpan BioSciences, Inc.

CNF, NPHN

Western blot

Supportive data in Antibodypedia

Provider product page for LS-C747566

Antibody Data

Product number

LS-C747566

Provider

LifeSpan BioSciences, Inc.

Product name

NPHS1 / Nephrin Antibody LS-C747566

Provider product page

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LifeSpan BioSciences, Inc. (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using NPHS1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Supportive validation

Submitted by

LifeSpan BioSciences, Inc. (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using NPHS1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.