Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ABIN500330 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- Nephrin antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human Nephrin.
- Description
- Peptide affinity chromatography
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Epitope
- C-Term
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Molecular basis of glomerular permselectivity.
Unraveling the mechanisms of glomerular ultrafiltration: nephrin, a key component of the slit diaphragm.
Positionally cloned gene for a novel glomerular protein--nephrin--is mutated in congenital nephrotic syndrome.
Tryggvason K, Wartiovaara J
Current opinion in nephrology and hypertension 2001 Jul;10(4):543-9
Current opinion in nephrology and hypertension 2001 Jul;10(4):543-9
Unraveling the mechanisms of glomerular ultrafiltration: nephrin, a key component of the slit diaphragm.
Tryggvason K
Journal of the American Society of Nephrology : JASN 1999 Nov;10(11):2440-5
Journal of the American Society of Nephrology : JASN 1999 Nov;10(11):2440-5
Positionally cloned gene for a novel glomerular protein--nephrin--is mutated in congenital nephrotic syndrome.
Kestilä M, Lenkkeri U, Männikkö M, Lamerdin J, McCready P, Putaala H, Ruotsalainen V, Morita T, Nissinen M, Herva R, Kashtan CE, Peltonen L, Holmberg C, Olsen A, Tryggvason K
Molecular cell 1998 Mar;1(4):575-82
Molecular cell 1998 Mar;1(4):575-82
No comments: Submit comment
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis in mouse kidney tissue lysate using Nephrin antibody Cat.-No. AP30586PU-N at 1 ?g/ml in the (A) absence and (B) presence of blocking peptide.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunofluorescence in mouse kidney cells using Nephrin antibody Cat.-No. AP30586PU-N at 10 μg/ml.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry in mouse kidney tissue using Nephrin antibody Cat.-No. AP30586PU-N at 1 ?g/ml.
