PA5-79399

antibody from Invitrogen Antibodies

Targeting: HSD11B2 SDR9C3

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-79399

Provider

Invitrogen Antibodies

Product name

HSD11B2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

500 µg/mL

Storage

-20°C

References

Submitted references

Metabolomics analysis of follicular fluid coupled with oocyte aspiration reveals importance of glucocorticoids in primate periovulatory follicle competency.

Ravisankar S, Hanna CB, Brooks KE, Murphy MJ, Redmayne N, Ryu J, Kinchen JM, Chavez SL, Hennebold JD
Scientific reports 2021 Mar 22;11(1):6506

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 Glucocorticoids and the enzymes that metabolize glucocorticoids are present in the rhesus macaque periovulatory follicle. ( a ) LC-MS/MS analysis of cortisol and cortisone concentrations in the FF obtained from rhesus macaque follicles between pre- (0 h) and 36 h post-hCG administration. A statistically significant increase in cortisol (* p = 0.0137) and decrease in cortisone (* p = 0.0110), with a corresponding increase in the cortisol to cortisone ratio (* p = 0.0256), was observed following hCG injection. ( b ) IHC of HSD11B1 and ( c ) HSD11B2 immunolocalization in the rhesus macaque periovulatory follicle pre- (0 h) as well as 12 h, 24 h and 36 h post-hCG administration. The images shown are representative of N = 4 ovaries obtained from separate animals undergoing a COv protocol at each of the times indicated. Note the overall increase in HSD11B1, as well as a concomitant decrease in HSD11B2 expression, in the ovarian follicle prior to hCG administration and with increasing time after the ovulatory stimulus. O = oocyte, C = cumulus cells, A = antrum, T = theca cells, M = mural granulosa cells and NC = negative control.