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LearnPA5-27074
antibody from Invitrogen Antibodies
Targeting: LPAR3
Edg-7, EDG7, HOFNH30, LP-A3, LPA3, RP4-678I3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-27074 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LPAR3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: NT2D1, PC-3. Predicted reactivity: Mouse (100%), Rat (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references ZEB1-AS1/miR-133a-3p/LPAR3/EGFR axis promotes the progression of thyroid cancer by regulating PI3K/AKT/mTOR pathway.
Xia W, Jie W
Cancer cell international 2020;20:94
Cancer cell international 2020;20:94
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LPAR3 Polyclonal Antibody detects LPAR3 protein by Western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract. B. 30 µg PC-3 whole cell lysate/extract.10 % SDS-PAGE. LPAR3 Polyclonal Antibody (Product # PA5-27074) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LPAR3 Polyclonal Antibody detects EDG7 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. LPAR3 Polyclonal Antibody (Product # PA5-27074) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- LPAR3 Polyclonal Antibody detects EDG7 protein at cytosol on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. LPAR3 Polyclonal Antibody (Product # PA5-27074) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human testis, using EDG7 (Product # PA5-27074) antibody (20 µg/mL). Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Additional file 2: Figure S2. (A, B) Western blot assays of Fig. 4 j and Fig. 6 a were quantified respectively. ** P < 0.01.
