Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- AF857 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal XCR1/CCXCR1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human XCR1 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of XCR1 in NS0 Mouse Cell Line Transfected with Human XCR-1 and eGFP by Flow Cytometry. NS0 mouse myeloma cell line transfected with human XCR-1 and eGFP was stained with and either (A) Goat Anti-Human XCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF857) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of XCR1 in Human Monocytes by Flow Cytometry. Human peripheral blood monocytes were treated for 16 hours with 1 μg/mL LPS then stained with Goat Anti-Human XCR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF857, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).