Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- ABIN1881054 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Angiomotin (AMOT) (pTyr599) antibody
- Antibody type
- Polyclonal
- Antigen
- This AMOT Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y599 of human AMOT.
- Description
- This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- pTyr599
- Antibody clone number
- RB41443
- Vial size
- 400 μL
- Concentration
- 0.5 mg/mL
Submitted references Amot recognizes a juxtanuclear endocytic recycling compartment via a novel lipid binding domain.
Human angiomotin-like 1 associates with an angiomotin protein complex through its coiled-coil domain and induces the remodeling of the actin cytoskeleton.
Angiomotin-like protein 1 controls endothelial polarity and junction stability during sprouting angiogenesis.
Heller B, Adu-Gyamfi E, Smith-Kinnaman W, Babbey C, Vora M, Xue Y, Bittman R, Stahelin RV, Wells CD
The Journal of biological chemistry 2010 Apr 16;285(16):12308-20
The Journal of biological chemistry 2010 Apr 16;285(16):12308-20
Human angiomotin-like 1 associates with an angiomotin protein complex through its coiled-coil domain and induces the remodeling of the actin cytoskeleton.
Gagné V, Moreau J, Plourde M, Lapointe M, Lord M, Gagnon E, Fernandes MJ
Cell motility and the cytoskeleton 2009 Sep;66(9):754-68
Cell motility and the cytoskeleton 2009 Sep;66(9):754-68
Angiomotin-like protein 1 controls endothelial polarity and junction stability during sprouting angiogenesis.
Zheng Y, Vertuani S, Nyström S, Audebert S, Meijer I, Tegnebratt T, Borg JP, Uhlén P, Majumdar A, Holmgren L
Circulation research 2009 Jul 31;105(3):260-70
Circulation research 2009 Jul 31;105(3):260-70
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Dot blot analysis of AMOT Antibody (Phospho Y599) Phospho-specific Pab (ABIN1881054) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6 μg per mL.