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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AGR-041-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GPR4 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4)kidney membranes: - 1,2. Anti-GPR4 (extracellular) Antibody (#AGR-041), (1:200).3,4. Anti-GPR4 (extracellular) Antibody , preincubated with GPR4 (extracellular) Blocking Peptide (#BLP-GR041).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat dorsal root ganglion lysate: - 1. Anti-GPR4 (extracellular) Antibody (#AGR-041), (1:200). 2. Anti-GPR4 (extracellular) Antibody , preincubated with GPR4 (extracellular) Blocking Peptide (#BLP-GR041).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4)kidney membranes: - 1,2. Anti-GPR4 (extracellular) Antibody (#AGR-041), (1:200).3,4. Anti-GPR4 (extracellular) Antibody , preincubated with GPR4 (extracellular) Blocking Peptide (#BLP-GR041).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat dorsal root ganglion lysate: - 1. Anti-GPR4 (extracellular) Antibody (#AGR-041), (1:200). 2. Anti-GPR4 (extracellular) Antibody , preincubated with GPR4 (extracellular) Blocking Peptide (#BLP-GR041).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression ofGPR4inrat dorsal raphe nucleus (DRN). Immunohistochemicalstaining of perfusion-fixed frozenratbrainsections with Anti-GPR4 (extracellular) Antibody (#AGR-041), (1:400), followed bygoat Anti-rabbit-Alexa-488.A.GPR4immunoreactivity (green)appears incells of both medial and lateral DRN (arrows).B. Pre-incubation of the Antibody with GPR4 (extracellular) Blocking Peptide (#BLP-GR041),suppressed staining. Cell nuclei are stained with DAPI (blue).LDRN = lateral DRN, MDRN = medial DRN.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of GPR4 in live intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-GPR4 (extracellular) Antibody (#AGR-041), (2.5 µg) + goat- Anti-rabbit-FITC.
