AF1589

antibody from R&D Systems

Targeting: SOST DAND6, VBCH

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1589

Provider

R&D Systems

Product name

Mouse SOST/Sclerostin Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse SOST/Sclerostin in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SOST is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

NP_077769


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Cathepsin K-deficient osteocytes prevent lactation-induced bone loss and parathyroid hormone suppression.

Lotinun S, Ishihara Y, Nagano K, Kiviranta R, Carpentier VT, Neff L, Parkman V, Ide N, Hu D, Dann P, Brooks D, Bouxsein ML, Wysolmerski J, Gori F, Baron R
The Journal of clinical investigation 2019 May 21;129(8):3058-3071

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TG-interacting factor 1 (Tgif1)-deficiency attenuates bone remodeling and blunts the anabolic response to parathyroid hormone.

Saito H, Gasser A, Bolamperti S, Maeda M, Matthies L, Jähn K, Long CL, Schlüter H, Kwiatkowski M, Saini V, Pajevic PD, Bellido T, van Wijnen AJ, Mohammad KS, Guise TA, Taipaleenmäki H, Hesse E
Nature communications 2019 Mar 22;10(1):1354

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Forceful mastication activates osteocytes and builds a stout jawbone.

Inoue M, Ono T, Kameo Y, Sasaki F, Ono T, Adachi T, Nakashima T
Scientific reports 2019 Mar 20;9(1):4404

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Relaxin 2 carried by magnetically directed liposomes accelerates rat midpalatal suture expansion and subsequent new bone formation.

Kamimoto H, Kobayashi Y, Moriyama K
Bone reports 2019 Jun;10:100202

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FasL Modulates Expression of Mmp2 in Osteoblasts.

Svandova E, Vesela B, Lesot H, Sadoine J, Poliard A, Matalova E
Frontiers in physiology 2018;9:1314

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Mechanically induced Ca²⁺ oscillations in osteocytes release extracellular vesicles and enhance bone formation.

Morrell AE, Brown GN, Robinson ST, Sattler RL, Baik AD, Zhen G, Cao X, Bonewald LF, Jin W, Kam LC, Guo XE
Bone research 2018;6:6

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Sclerostin is upregulated in the early stage of chondrogenic differentiation, but not required in endochondral ossification in vitro.

Yamaguchi Y, Kumagai K, Imai S, Miyatake K, Saito T
PloS one 2018;13(8):e0201839

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Simvastatin attenuates tibial bone loss in rats with type 1 diabetes and periodontitis.

Kim AR, Kim JH, Kim A, Sohn Y, Cha JH, Bak EJ, Yoo YJ
Journal of translational medicine 2018 Nov 9;16(1):306

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Bone adaptation in response to treadmill exercise in young and adult mice.

Gardinier JD, Rostami N, Juliano L, Zhang C
Bone reports 2018 Jun;8:29-37

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The Deletion of Hdac4 in Mouse Osteoblasts Influences Both Catabolic and Anabolic Effects in Bone.

Nakatani T, Chen T, Johnson J, Westendorf JJ, Partridge NC
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2018 Jul;33(7):1362-1375

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Zoledronate promotes bone formation by blocking osteocyte-osteoblast communication during bone defect healing.

Cui P, Liu H, Sun J, Amizuka N, Sun Q, Li M
Histology and histopathology 2018 Jan;33(1):89-99

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FGF2 crosstalk with Wnt signaling in mediating the anabolic action of PTH on bone formation.

Xiao L, Fei Y, Hurley MM
Bone reports 2018 Dec;9:136-144

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Transient peak-strain matching partially recovers the age-impaired mechanoadaptive cortical bone response.

Javaheri B, Carriero A, Wood M, De Souza R, Lee PD, Shefelbine S, Pitsillides AA
Scientific reports 2018 Apr 27;8(1):6636

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Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone.

Sakurai A, Hasegawa T, Kudo A, Shen Z, Nagai T, Abe M, Yoshida T, Hongo H, Yamamoto T, Yamamoto T, Oda K, Freitas PHL, Li M, Sano H, Amizuka N
Biomedical research (Tokyo, Japan) 2017;38(4):257-267

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Sclerostin Promotes Bone Remodeling in the Process of Tooth Movement.

Shu R, Bai D, Sheu T, He Y, Yang X, Xue C, He Y, Zhao M, Han X
PloS one 2017;12(1):e0167312

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Gsα Controls Cortical Bone Quality by Regulating Osteoclast Differentiation via cAMP/PKA and β-Catenin Pathways.

Ramaswamy G, Kim H, Zhang D, Lounev V, Wu JY, Choi Y, Kaplan FS, Pignolo RJ, Shore EM
Scientific reports 2017 Mar 24;7:45140

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Suppression of Sclerostin Alleviates Radiation-Induced Bone Loss by Protecting Bone-Forming Cells and Their Progenitors Through Distinct Mechanisms.

Chandra A, Lin T, Young T, Tong W, Ma X, Tseng WJ, Kramer I, Kneissel M, Levine MA, Zhang Y, Cengel K, Liu XS, Qin L
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2017 Feb;32(2):360-372

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Inflammatory Bowel Disease in a Rodent Model Alters Osteocyte Protein Levels Controlling Bone Turnover.

Metzger CE, Narayanan A, Zawieja DC, Bloomfield SA
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2017 Apr;32(4):802-813

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Distorted Patterns of Dentinogenesis and Eruption in Msx2 Null Mutants: Involvement of Sost/Sclerostin.

Amri N, Djolé SX, Petit S, Babajko S, Coudert AE, Castaneda B, Simon S, Berdal A
The American journal of pathology 2016 Oct;186(10):2577-87

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Short-term intermittent administration of parathyroid hormone facilitates osteogenesis by different mechanisms in cancellous and cortical bone.

Ogura K, Iimura T, Makino Y, Sugie-Oya A, Takakura A, Takao-Kawabata R, Ishizuya T, Moriyama K, Yamaguchi A
Bone reports 2016 Dec;5:7-14

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Parathyroid Hormone Induces Bone Cell Motility and Loss of Mature Osteocyte Phenotype through L-Calcium Channel Dependent and Independent Mechanisms.

Prideaux M, Dallas SL, Zhao N, Johnsrud ED, Veno PA, Guo D, Mishina Y, Harris SE, Bonewald LF
PloS one 2015;10(5):e0125731

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The Wnt Inhibitor Sclerostin Is Up-regulated by Mechanical Unloading in Osteocytes in Vitro.

Spatz JM, Wein MN, Gooi JH, Qu Y, Garr JL, Liu S, Barry KJ, Uda Y, Lai F, Dedic C, Balcells-Camps M, Kronenberg HM, Babij P, Pajevic PD
The Journal of biological chemistry 2015 Jul 3;290(27):16744-58

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Heavy Metal Ion Regulation of Gene Expression: MECHANISMS BY WHICH LEAD INHIBITS OSTEOBLASTIC BONE-FORMING ACTIVITY THROUGH MODULATION OF THE Wnt/β-CATENIN SIGNALING PATHWAY.

Beier EE, Sheu TJ, Dang D, Holz JD, Ubayawardena R, Babij P, Puzas JE
The Journal of biological chemistry 2015 Jul 17;290(29):18216-26

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Osteocytes mediate the anabolic actions of canonical Wnt/β-catenin signaling in bone.

Tu X, Delgado-Calle J, Condon KW, Maycas M, Zhang H, Carlesso N, Taketo MM, Burr DB, Plotkin LI, Bellido T
Proceedings of the National Academy of Sciences of the United States of America 2015 Feb 3;112(5):E478-86

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Androgens inhibit the osteogenic response to mechanical loading in adult male mice.

Sinnesael M, Laurent MR, Jardi F, Dubois V, Deboel L, Delisser P, Behets GJ, D'Haese PC, Carmeliet G, Claessens F, Vanderschueren D
Endocrinology 2015 Apr;156(4):1343-53

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Disrupted bone remodeling leads to cochlear overgrowth and hearing loss in a mouse model of fibrous dysplasia.

Akil O, Hall-Glenn F, Chang J, Li A, Chang W, Lustig LR, Alliston T, Hsiao EC
PloS one 2014;9(5):e94989

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The osteoblast to osteocyte transition: epigenetic changes and response to the vitamin D3 hormone.

St John HC, Bishop KA, Meyer MB, Benkusky NA, Leng N, Kendziorski C, Bonewald LF, Pike JW
Molecular endocrinology (Baltimore, Md.) 2014 Jul;28(7):1150-65

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Inhibition of GSK-3β rescues the impairments in bone formation and mechanical properties associated with fracture healing in osteoblast selective connexin 43 deficient mice.

Loiselle AE, Lloyd SA, Paul EM, Lewis GS, Donahue HJ
PloS one 2013;8(11):e81399

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Matrix metalloproteinase-13 is required for osteocytic perilacunar remodeling and maintains bone fracture resistance.

Tang SY, Herber RP, Ho SP, Alliston T
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2012 Sep;27(9):1936-50

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miR-218 directs a Wnt signaling circuit to promote differentiation of osteoblasts and osteomimicry of metastatic cancer cells.

Hassan MQ, Maeda Y, Taipaleenmaki H, Zhang W, Jafferji M, Gordon JA, Li Z, Croce CM, van Wijnen AJ, Stein JL, Stein GS, Lian JB
The Journal of biological chemistry 2012 Dec 7;287(50):42084-92

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Repression of osteocyte Wnt/β-catenin signaling is an early event in the progression of renal osteodystrophy.

Sabbagh Y, Graciolli FG, O'Brien S, Tang W, dos Reis LM, Ryan S, Phillips L, Boulanger J, Song W, Bracken C, Liu S, Ledbetter S, Dechow P, Canziani ME, Carvalho AB, Jorgetti V, Moyses RM, Schiavi SC
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2012 Aug;27(8):1757-72

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The appearance and modulation of osteocyte marker expression during calcification of vascular smooth muscle cells.

Zhu D, Mackenzie NC, Millán JL, Farquharson C, MacRae VE
PloS one 2011;6(5):e19595

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Parathyroid hormone receptor signaling in osteocytes increases the expression of fibroblast growth factor-23 in vitro and in vivo.

Rhee Y, Bivi N, Farrow E, Lezcano V, Plotkin LI, White KE, Bellido T
Bone 2011 Oct;49(4):636-43

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Conditional deletion of Bmpr1a in differentiated osteoclasts increases osteoblastic bone formation, increasing volume of remodeling bone in mice.

Okamoto M, Murai J, Imai Y, Ikegami D, Kamiya N, Kato S, Mishina Y, Yoshikawa H, Tsumaki N
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2011 Oct;26(10):2511-22

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Distinct modes of inhibition by sclerostin on bone morphogenetic protein and Wnt signaling pathways.

Krause C, Korchynskyi O, de Rooij K, Weidauer SE, de Gorter DJ, van Bezooijen RL, Hatsell S, Economides AN, Mueller TD, Löwik CW, ten Dijke P
The Journal of biological chemistry 2010 Dec 31;285(53):41614-26

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Localization of SOST/sclerostin in cementocytes in vivo and in mineralizing periodontal ligament cells in vitro.

Jäger A, Götz W, Lossdörfer S, Rath-Deschner B
Journal of periodontal research 2010 Apr;45(2):246-54

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Parathyroid hormone signaling through low-density lipoprotein-related protein 6.

Wan M, Yang C, Li J, Wu X, Yuan H, Ma H, He X, Nie S, Chang C, Cao X
Genes & development 2008 Nov 1;22(21):2968-79

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Control of the SOST bone enhancer by PTH using MEF2 transcription factors.

Leupin O, Kramer I, Collette NM, Loots GG, Natt F, Kneissel M, Keller H
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2007 Dec;22(12):1957-67

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse SOST/Sclerostin by Western Blot. Western blot shows lysates of human bone marrow and mouse bone marrow. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOST/Sclerostin at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

SOST/Sclerostin in Mouse Embryo. SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (adrenal gland) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

SOST/Sclerostin in Mouse Embryo. SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse SOST/Sclerostin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.