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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunohistochemistry [1]
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- Product number
- BAF1589 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse SOST/Sclerostin Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects SOST/Sclerostin in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human SOST is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
NP_077769- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Differential involvement of Wnt signaling in Bmp regulation of cancellous versus periosteal bone growth.
Immunolocalization of osteocyte-derived molecules during bone fracture healing of mouse ribs.
Loss of Gsα in the Postnatal Skeleton Leads to Low Bone Mass and a Blunted Response to Anabolic Parathyroid Hormone Therapy.
The Wnt Inhibitor Sclerostin Is Up-regulated by Mechanical Unloading in Osteocytes in Vitro.
Gsα enhances commitment of mesenchymal progenitors to the osteoblast lineage but restrains osteoblast differentiation in mice.
He G, Shi Y, Lim J, Bellido T, Ni J, Long F
Bone research 2017;5:17016
Bone research 2017;5:17016
Immunolocalization of osteocyte-derived molecules during bone fracture healing of mouse ribs.
Liu Z, Yamamoto T, Hasegawa T, Hongo H, Tsuboi K, Tsuchiya E, Haraguchi M, Abe M, Freitas PH, Kudo A, Oda K, Li M, Amizuka N
Biomedical research (Tokyo, Japan) 2016;37(2):141-51
Biomedical research (Tokyo, Japan) 2016;37(2):141-51
Loss of Gsα in the Postnatal Skeleton Leads to Low Bone Mass and a Blunted Response to Anabolic Parathyroid Hormone Therapy.
Sinha P, Aarnisalo P, Chubb R, Poulton IJ, Guo J, Nachtrab G, Kimura T, Swami S, Saeed H, Chen M, Weinstein LS, Schipani E, Sims NA, Kronenberg HM, Wu JY
The Journal of biological chemistry 2016 Jan 22;291(4):1631-42
The Journal of biological chemistry 2016 Jan 22;291(4):1631-42
The Wnt Inhibitor Sclerostin Is Up-regulated by Mechanical Unloading in Osteocytes in Vitro.
Spatz JM, Wein MN, Gooi JH, Qu Y, Garr JL, Liu S, Barry KJ, Uda Y, Lai F, Dedic C, Balcells-Camps M, Kronenberg HM, Babij P, Pajevic PD
The Journal of biological chemistry 2015 Jul 3;290(27):16744-58
The Journal of biological chemistry 2015 Jul 3;290(27):16744-58
Gsα enhances commitment of mesenchymal progenitors to the osteoblast lineage but restrains osteoblast differentiation in mice.
Wu JY, Aarnisalo P, Bastepe M, Sinha P, Fulzele K, Selig MK, Chen M, Poulton IJ, Purton LE, Sims NA, Weinstein LS, Kronenberg HM
The Journal of clinical investigation 2011 Sep;121(9):3492-504
The Journal of clinical investigation 2011 Sep;121(9):3492-504
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SOST/Sclerostin in Mouse Embryo. SOST/Sclerostin was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Mouse SOST/Sclerostin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
