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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP13235PU-N - Provider product page
- Provider
- OriGene
- Product name
- Sclerostin (SOST) (N-term) rabbit polyclonal antibody, Purified
- Antibody type
- Polyclonal
- Description
- Sclerostin (SOST) (N-term) rabbit polyclonal antibody, Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SOST
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml (lot specific)
Submitted references Osteocytes mediate the anabolic actions of canonical Wnt/β-catenin signaling in bone.
Tu X, Delgado-Calle J, Condon KW, Maycas M, Zhang H, Carlesso N, Taketo MM, Burr DB, Plotkin LI, Bellido T
Proceedings of the National Academy of Sciences of the United States of America 2015 Feb 3;112(5):E478-86
Proceedings of the National Academy of Sciences of the United States of America 2015 Feb 3;112(5):E478-86
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of anti-SOST Antibody (N-term) Pab in mouse liver tissue lysates (35ug/lane). SOST (arrow) was detected using the purified Pab (1:60 dilution).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from human lung, mouse lung tissue lysate (from left to right), using SOST Antibody (N-term)(?). ?was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from Hela cell line, human lung, mouse lung, mouse liver tissue lysate (from left to right), using SOST Antibody (N-term) . was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from mouse liver, mouse lung, rat kidney tissue lysate (from left to right), using SOST Antibody (N-term). was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SOST antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
- Validation comment
- IHC
