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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AP8478a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP8478a, RRID:AB_2095162
- Product name
- DUSP3 Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 2 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Vaccinia H1-related phosphatase is a phosphatase of ErbB receptors and is down-regulated in non-small cell lung cancer.
Wang JY, Yeh CL, Chou HC, Yang CH, Fu YN, Chen YT, Cheng HW, Huang CY, Liu HP, Huang SF, Chen YR
The Journal of biological chemistry 2011 Mar 25;286(12):10177-84
The Journal of biological chemistry 2011 Mar 25;286(12):10177-84
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of anti-DUSP3 Pab (Cat. #AP8478a) in SK-BR-3 cell line lysate.DUSP3(arrow) was detected using the purified Pab.
- Primary Ab dilution
- 1:1000
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of DUSP3 (arrow) using rabbit polyclonal DUSP3 Antibody (E6) (Cat. #AP8478a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DUSP3 gene.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
