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- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AF539 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-13 R alpha 2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IL-13 R alpha 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) IL-13 R alpha 2 is observed and less than 1% cross-reactivity with rhIL-5R alpha , rhIL-5R beta , rhIL-9 R, and rhIL-4 R is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O88786- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Unexpected involvement of IL-13 signalling via a STAT6 independent mechanism during murine IgG2a development following viral vaccination.
Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice.
GdCl3 Attenuates Schistosomiasis japonicum Egg-Induced Granulomatosis Accompanied by Decreased Macrophage Infiltration in Murine Liver.
Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo.
Loss of pro-apoptotic Bim promotes accumulation of pulmonary T lymphocytes and enhances allergen-induced goblet cell metaplasia.
Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
Impact of interleukin-13 responsiveness on the synthetic and proliferative properties of Th1- and Th2-type pulmonary granuloma fibroblasts.
Hamid MA, Jackson RJ, Roy S, Khanna M, Ranasinghe C
European journal of immunology 2018 Jul;48(7):1153-1163
European journal of immunology 2018 Jul;48(7):1153-1163
Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice.
Sezaki T, Hirata Y, Hagiwara T, Kawamura YI, Okamura T, Takanashi R, Nakano K, Tamura-Nakano M, Burkly LC, Dohi T
World journal of gastroenterology 2017 Apr 7;23(13):2294-2307
World journal of gastroenterology 2017 Apr 7;23(13):2294-2307
GdCl3 Attenuates Schistosomiasis japonicum Egg-Induced Granulomatosis Accompanied by Decreased Macrophage Infiltration in Murine Liver.
Zheng S, Lu Q, Xu Y, Wang X, Shen J, Wang W
PloS one 2015;10(8):e0132222
PloS one 2015;10(8):e0132222
Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo.
Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK
The Journal of allergy and clinical immunology 2008 Sep;122(3):625-32
The Journal of allergy and clinical immunology 2008 Sep;122(3):625-32
Loss of pro-apoptotic Bim promotes accumulation of pulmonary T lymphocytes and enhances allergen-induced goblet cell metaplasia.
Pierce J, Rir-Sima-Ah J, Estrada I, Wilder J, Strasser A, Tesfaigzi Y
American journal of physiology. Lung cellular and molecular physiology 2006 Nov;291(5):L862-70
American journal of physiology. Lung cellular and molecular physiology 2006 Nov;291(5):L862-70
Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK
Journal of immunology (Baltimore, Md. : 1950) 2006 Jun 15;176(12):7495-501
Journal of immunology (Baltimore, Md. : 1950) 2006 Jun 15;176(12):7495-501
Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK
Journal of immunology (Baltimore, Md. : 1950) 2006 Jun 15;176(12):7495-501
Journal of immunology (Baltimore, Md. : 1950) 2006 Jun 15;176(12):7495-501
Impact of interleukin-13 responsiveness on the synthetic and proliferative properties of Th1- and Th2-type pulmonary granuloma fibroblasts.
Jakubzick C, Choi ES, Kunkel SL, Joshi BH, Puri RK, Hogaboam CM
The American journal of pathology 2003 May;162(5):1475-86
The American journal of pathology 2003 May;162(5):1475-86
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-13 R alpha 2 in Mouse Splenocytes. IL-13 R alpha 2 was detected in immersion fixed mouse splenocytes using Mouse IL-13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF539) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-13 R alpha 2 in Mouse Thymus. IL-13 R alpha 2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL-13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF539) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
