Antibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF146 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-13 R alpha 2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-13 R alpha 2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL-13 R alpha 2 is observed and less than 1% cross-reactivity with recombinant human IL-13 R alpha 1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q14627
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IL-13 receptors as possible therapeutic targets in diffuse intrinsic pontine glioma.
Trivalent CAR T cells overcome interpatient antigenic variability in glioblastoma.
Optimization of IL13Rα2-Targeted Chimeric Antigen Receptor T Cells for Improved Anti-tumor Efficacy against Glioblastoma.
IL4 Primes the Dynamics of Breast Cancer Progression via DUSP4 Inhibition.
Cigarette smoke extracts induce overexpression of the proto-oncogenic gene interleukin-13 receptor α2 through activation of the PKA-CREB signaling pathway to trigger malignant transformation of lung vascular endothelial cells and angiogenesis.
IL-13Rα2 uses TMEM219 in chitinase 3-like-1-induced signalling and effector responses.
Tandem CAR T cells targeting HER2 and IL13Rα2 mitigate tumor antigen escape.
Role of IL13RA2 in Sunitinib Resistance in Clear Cell Renal Cell Carcinoma.
Bioactivity and Safety of IL13Rα2-Redirected Chimeric Antigen Receptor CD8+ T Cells in Patients with Recurrent Glioblastoma.
IL-13Rα2 mediates PNR-induced migration and metastasis in ERα-negative breast cancer.
Expression of surfactant protein D in airways of asthmatics and interleukin-13 modulation of surfactant protein D in human models of airway epithelium.
Cytokine induction of VCAM-1 but not IL13Rα2 on glioma cells: a tale of two antibodies.
Absence of a role for interleukin-13 in inflammatory bowel disease.
Combinational targeting offsets antigen escape and enhances effector functions of adoptively transferred T cells in glioblastoma.
Interleukin-13 displaying retargeted oncolytic measles virus strains have significant activity against gliomas with improved specificity.
Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo.
Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay.
Effect of IL-13 receptor alpha2 levels on the biological activity of IL-13 variant R110Q.
IL-13 receptor alpha 2: a regulator of IL-13 and IL-4 signal transduction in primary human fibroblasts.
Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
Protection of human conjunctival fibroblasts from NO-induced apoptosis by interleukin-4 or interleukin-13.
Soluble interleukin-13Ralpha2 decoy receptor inhibits Hodgkin's lymphoma growth in vitro and in vivo.
Soluble interleukin-13Ralpha2 decoy receptor inhibits Hodgkin's lymphoma growth in vitro and in vivo.
Berlow NE, Svalina MN, Quist MJ, Settelmeyer TP, Zherebitskiy V, Kogiso M, Qi L, Du Y, Hawkins CE, Hulleman E, Li XN, Gultekin SH, Keller C
PloS one 2018;13(4):e0193565
PloS one 2018;13(4):e0193565
Trivalent CAR T cells overcome interpatient antigenic variability in glioblastoma.
Bielamowicz K, Fousek K, Byrd TT, Samaha H, Mukherjee M, Aware N, Wu MF, Orange JS, Sumazin P, Man TK, Joseph SK, Hegde M, Ahmed N
Neuro-oncology 2018 Mar 27;20(4):506-518
Neuro-oncology 2018 Mar 27;20(4):506-518
Optimization of IL13Rα2-Targeted Chimeric Antigen Receptor T Cells for Improved Anti-tumor Efficacy against Glioblastoma.
Brown CE, Aguilar B, Starr R, Yang X, Chang WC, Weng L, Chang B, Sarkissian A, Brito A, Sanchez JF, Ostberg JR, D'Apuzzo M, Badie B, Barish ME, Forman SJ
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Jan 3;26(1):31-44
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Jan 3;26(1):31-44
IL4 Primes the Dynamics of Breast Cancer Progression via DUSP4 Inhibition.
Gaggianesi M, Turdo A, Chinnici A, Lipari E, Apuzzo T, Benfante A, Sperduti I, Di Franco S, Meraviglia S, Lo Presti E, Dieli F, Caputo V, Militello G, Vieni S, Stassi G, Todaro M
Cancer research 2017 Jun 15;77(12):3268-3279
Cancer research 2017 Jun 15;77(12):3268-3279
Cigarette smoke extracts induce overexpression of the proto-oncogenic gene interleukin-13 receptor α2 through activation of the PKA-CREB signaling pathway to trigger malignant transformation of lung vascular endothelial cells and angiogenesis.
Meng M, Liao H, Zhang B, Pan Y, Kong Y, Liu W, Yang P, Huo Z, Cao Z, Zhou Q
Cellular signalling 2017 Feb;31:15-25
Cellular signalling 2017 Feb;31:15-25
IL-13Rα2 uses TMEM219 in chitinase 3-like-1-induced signalling and effector responses.
Lee CM, He CH, Nour AM, Zhou Y, Ma B, Park JW, Kim KH, Dela Cruz C, Sharma L, Nasr ML, Modis Y, Lee CG, Elias JA
Nature communications 2016 Sep 15;7:12752
Nature communications 2016 Sep 15;7:12752
Tandem CAR T cells targeting HER2 and IL13Rα2 mitigate tumor antigen escape.
Hegde M, Mukherjee M, Grada Z, Pignata A, Landi D, Navai SA, Wakefield A, Fousek K, Bielamowicz K, Chow KK, Brawley VS, Byrd TT, Krebs S, Gottschalk S, Wels WS, Baker ML, Dotti G, Mamonkin M, Brenner MK, Orange JS, Ahmed N
The Journal of clinical investigation 2016 Aug 1;126(8):3036-52
The Journal of clinical investigation 2016 Aug 1;126(8):3036-52
Role of IL13RA2 in Sunitinib Resistance in Clear Cell Renal Cell Carcinoma.
Shibasaki N, Yamasaki T, Kanno T, Arakaki R, Sakamoto H, Utsunomiya N, Inoue T, Tsuruyama T, Nakamura E, Ogawa O, Kamba T
PloS one 2015;10(6):e0130980
PloS one 2015;10(6):e0130980
Bioactivity and Safety of IL13Rα2-Redirected Chimeric Antigen Receptor CD8+ T Cells in Patients with Recurrent Glioblastoma.
Brown CE, Badie B, Barish ME, Weng L, Ostberg JR, Chang WC, Naranjo A, Starr R, Wagner J, Wright C, Zhai Y, Bading JR, Ressler JA, Portnow J, D'Apuzzo M, Forman SJ, Jensen MC
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Sep 15;21(18):4062-72
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Sep 15;21(18):4062-72
IL-13Rα2 mediates PNR-induced migration and metastasis in ERα-negative breast cancer.
Zhao Z, Wang L, Xu W
Oncogene 2015 Mar 19;34(12):1596-607
Oncogene 2015 Mar 19;34(12):1596-607
Expression of surfactant protein D in airways of asthmatics and interleukin-13 modulation of surfactant protein D in human models of airway epithelium.
Xu J, Singhera GK, Dorscheid DR
Respiratory research 2015 Feb 15;16:26
Respiratory research 2015 Feb 15;16:26
Cytokine induction of VCAM-1 but not IL13Rα2 on glioma cells: a tale of two antibodies.
Mahadev V, Starr R, Wright SL, Martinez C, Jensen MC, Barish ME, Forman SJ, Brown CE
PloS one 2014;9(5):e95123
PloS one 2014;9(5):e95123
Absence of a role for interleukin-13 in inflammatory bowel disease.
Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque SS, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza GR, MacDonald TT
European journal of immunology 2014 Feb;44(2):370-85
European journal of immunology 2014 Feb;44(2):370-85
Combinational targeting offsets antigen escape and enhances effector functions of adoptively transferred T cells in glioblastoma.
Hegde M, Corder A, Chow KK, Mukherjee M, Ashoori A, Kew Y, Zhang YJ, Baskin DS, Merchant FA, Brawley VS, Byrd TT, Krebs S, Wu MF, Liu H, Heslop HE, Gottschalk S, Yvon E, Ahmed N
Molecular therapy : the journal of the American Society of Gene Therapy 2013 Nov;21(11):2087-101
Molecular therapy : the journal of the American Society of Gene Therapy 2013 Nov;21(11):2087-101
Interleukin-13 displaying retargeted oncolytic measles virus strains have significant activity against gliomas with improved specificity.
Allen C, Paraskevakou G, Iankov I, Giannini C, Schroeder M, Sarkaria J, Schroeder M, Puri RK, Russell SJ, Galanis E
Molecular therapy : the journal of the American Society of Gene Therapy 2008 Sep;16(9):1556-64
Molecular therapy : the journal of the American Society of Gene Therapy 2008 Sep;16(9):1556-64
Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo.
Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK
The Journal of allergy and clinical immunology 2008 Sep;122(3):625-32
The Journal of allergy and clinical immunology 2008 Sep;122(3):625-32
Development of an in vitro potency bioassay for therapeutic IL-13 antagonists: the A-549 cell bioassay.
Miller R, Sadhukhan R, Wu C
Journal of immunological methods 2008 May 20;334(1-2):134-41
Journal of immunological methods 2008 May 20;334(1-2):134-41
Effect of IL-13 receptor alpha2 levels on the biological activity of IL-13 variant R110Q.
Andrews AL, Bucchieri F, Arima K, Izuhara K, Holgate ST, Davies DE, Holloway JW
The Journal of allergy and clinical immunology 2007 Jul;120(1):91-7
The Journal of allergy and clinical immunology 2007 Jul;120(1):91-7
IL-13 receptor alpha 2: a regulator of IL-13 and IL-4 signal transduction in primary human fibroblasts.
Andrews AL, Nasir T, Bucchieri F, Holloway JW, Holgate ST, Davies DE
The Journal of allergy and clinical immunology 2006 Oct;118(4):858-65
The Journal of allergy and clinical immunology 2006 Oct;118(4):858-65
Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK
Journal of immunology (Baltimore, Md. : 1950) 2006 Jun 15;176(12):7495-501
Journal of immunology (Baltimore, Md. : 1950) 2006 Jun 15;176(12):7495-501
Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling.
Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK
Journal of immunology (Baltimore, Md. : 1950) 2006 Jun 15;176(12):7495-501
Journal of immunology (Baltimore, Md. : 1950) 2006 Jun 15;176(12):7495-501
Protection of human conjunctival fibroblasts from NO-induced apoptosis by interleukin-4 or interleukin-13.
Fujitsu Y, Fukuda K, Kimura K, Seki K, Kumagai N, Nishida T
Investigative ophthalmology & visual science 2005 Mar;46(3):797-802
Investigative ophthalmology & visual science 2005 Mar;46(3):797-802
Soluble interleukin-13Ralpha2 decoy receptor inhibits Hodgkin's lymphoma growth in vitro and in vivo.
Trieu Y, Wen XY, Skinnider BF, Bray MR, Li Z, Claudio JO, Masih-Khan E, Zhu YX, Trudel S, McCart JA, Mak TW, Stewart AK
Cancer research 2004 May 1;64(9):3271-5
Cancer research 2004 May 1;64(9):3271-5
Soluble interleukin-13Ralpha2 decoy receptor inhibits Hodgkin's lymphoma growth in vitro and in vivo.
Trieu Y, Wen XY, Skinnider BF, Bray MR, Li Z, Claudio JO, Masih-Khan E, Zhu YX, Trudel S, McCart JA, Mak TW, Stewart AK
Cancer research 2004 May 1;64(9):3271-5
Cancer research 2004 May 1;64(9):3271-5
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-13 R alpha 2 by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue and human testis tissue, loaded at 0.2 mg/mL. A specific band was detected for IL-13 R alpha 2 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Human IL-13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-13 R alpha 2 by Western Blot. Western blot shows lysates of human placenta tissue and human testis tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human IL-13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-13 R alpha 2 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-13 R alpha 2 in Human Prostate Cancer Tissue. IL-13 R alpha 2 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human IL-13 R alpha 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF146) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.