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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX81089 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81089, RRID:AB_11170385
- Product name
- SEMA3E antibody, C-term
- Antibody type
- Polyclonal
- Host
- Rabbit
- Vial size
- 100 µg
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of NCF1C Antibody (C-term) (GTX81089) in T47D cell line lysates (35ug/lane). NCF1C (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NCF1C Antibody (C-term)(GTX81089), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- NCF1C Antibody (C-term) (GTX81089) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
