- PA5-27399 - Invitrogen Antibodies ADK antibody

Submitted references Role of Adenosine Kinase in Sphingosine-1-Phosphate Receptor 1-Induced Mechano-Hypersensitivities.

Lauro F, Giancotti LA, Kolar G, Harada CM, Harmon TA, Garrett TJ, Salvemini D
Cellular and molecular neurobiology 2022 Nov;42(8):2909-2918

Adenosine Kinase Isoforms in the Developing Rat Hippocampus after LiCl/Pilocarpine Status Epilepticus.

Fábera P, Uttl L, Kubová H, Tsenov G, Mareš P
International journal of molecular sciences 2022 Feb 24;23(5)

Chronic Morphine-Induced Changes in Signaling at the A(3) Adenosine Receptor Contribute to Morphine-Induced Hyperalgesia, Tolerance, and Withdrawal.

Doyle TM, Largent-Milnes TM, Chen Z, Staikopoulos V, Esposito E, Dalgarno R, Fan C, Tosh DK, Cuzzocrea S, Jacobson KA, Trang T, Hutchinson MR, Bennett GJ, Vanderah TW, Salvemini D
The Journal of pharmacology and experimental therapeutics 2020 Aug;374(2):331-341

Chemotherapy-induced pain is promoted by enhanced spinal adenosine kinase levels through astrocyte-dependent mechanisms.

Wahlman C, Doyle TM, Little JW, Luongo L, Janes K, Chen Z, Esposito E, Tosh DK, Cuzzocrea S, Jacobson KA, Salvemini D
Pain 2018 Jun;159(6):1025-1034

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Adenosine kinase using 50 µg mouse liver lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Adenosine kinase polyclonal antibody (Product # PA5-27399) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Adenosine kinase using 50 µg rat liver lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Adenosine kinase polyclonal antibody (Product # PA5-27399) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Adenosine kinase using 30 µg of A) A431 and B) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Adenosine kinase polyclonal antibody (Product # PA5-27399) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Adenosine Kinase was performed by separating 50 µg of mouse tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Adenosine Kinase Polyclonal Antibody (Product # PA5-27399) at a dilution of 1:1500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Adenosine Kinase was performed by separating 50 µg of rat tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Adenosine Kinase Polyclonal Antibody (Product # PA5-27399) at a dilution of 1:1500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of Adenosine Kinase was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a Adenosine Kinase Polyclonal Antibody (Product # PA5-27399) at a dilution of 1:1,500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using Adenosine Kinase Polyclonal Antibody (Product # PA5-27399). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine Kinase Polyclonal Antibody (Product # PA5-27399) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of Adenosine Kinase was achieved by transfecting A-431 with Adenosine Kinase specific siRNAs (Silencer® select Product # s1081, s1080). Western blot analysis (Fig. a) was performed using whole cell extracts from the Adenosine Kinase knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Adenosine Kinase Polyclonal Antibody (Product # PA5-27399, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Adenosine Kinase.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane 1), A-431 (Lane 2), Hep G2 (Lane 3), MCF7 (Lane 4), tissue extracts (30 µg lysate) of Mouse Liver (Lane 5), Rat Liver (Lane 6), Mouse Kidney (Lane 7) and Rat Kidney (Lane 8) . The blot was probed with Anti-Adenosine Kinase Polyclonal Antibody (Product # PA5-27399, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 56 kDa band corresponding to Adenosine Kinase was detected across the cell lines and tissues tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Adenosine kinase in paraformaldehyde-fixed A431 cells using an Adenosine kinase polyclonal antibody (Product # PA5-27399) at a 1:200 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Adenosine Kinase was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Adenosine Kinase Polyclonal Antibody (Product # PA5-27399) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Adenosine Kinase Polyclonal Antibody detects Adenosine kinase protein at nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Adenosine Kinase Polyclonal Antibody (Product # PA5-27399) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Adenosine Kinase Polyclonal Antibody detects ADK protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ADK stained by Adenosine Kinase Polyclonal Antibody (Product # PA5-27399) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blotting of hippocampal homogenates showing the density of ADK isoforms-ADK-S (38.7 kDa) and ADK-L (40.5 kDa) during brain development in control rats ( A ) and corresponding age-matched LiCl/pilocarpine SE rats ( B ). ( A ) Abscissae from left to right: 7, 10, 12, 15, 18, 21, 25, 32, and 45-day-old rats in identical replicates. ( B ) Abscissae from left to right: 12-, 15-, and 32-day-old rats in identical replicates.

PA5-27399

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