Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ABIN953301 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
Submitted references PP2A T61 epsilon is an inhibitor of MAP4K3 in nutrient signaling to mTOR.
Yan L, Mieulet V, Burgess D, Findlay GM, Sully K, Procter J, Goris J, Janssens V, Morrice NA, Lamb RF
Molecular cell 2010 Mar 12;37(5):633-42
Molecular cell 2010 Mar 12;37(5):633-42
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of MAP4K3 Antibody Cat.-No AP52601PU-N in HepG2 cell line lysates (35ug/lane). MAP4K3 (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue reacted with MAP4K3 Antibody Cat.-No AP52601PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using MAP4K3 Antibody Cat.-No AP52601PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.