Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [2]
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Validation data
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- Product number
- PA5-77480 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LPAR5 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.85 mg/mL
- Storage
- -20°C
Submitted references TNRC6C-AS1 Promotes Thyroid Cancer Progression by Upregulating LPAR5 via miR-513c-5p.
Tong C, Wang C, Wang Y, Xiao X
Cancer management and research 2021;13:6141-6155
Cancer management and research 2021;13:6141-6155
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Chronic myelogenous leukemia MEG-01 (lanes 1 and 3) and K562 cell lysates (lanes 2 and 4) with LPAR5 (extracellular) polyclonal antibody (Product # PA5-77480) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Chronic myelogenous leukemia MEG-01 (lanes 1 and 3) and K562 cell lysates (lanes 2 and 4) with LPAR5 (extracellular) polyclonal antibody (Product # PA5-77480) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 LPAR5 is the target gene for miR-513c-5p. ( A ) The putative binding site of LPAR5 and miR-513c-5p is shown. ( B ) Luciferase activity was measured in SW579 and TPC-1 cells co-transfected with wild type (LPAR5-Wt) or mutant (LPAR5-Mut) LPAR5 and miR-513c-5p using the luciferase reporter assay. ** P < 0.001 vs miR-NC. ( C ) Biotinylated RNA pull-down assays were applied using Bio-NC and Bio-mimic. Relative enrichment of LPAR5 was measured by qRT-PCR. Bio-mimic, miR-513c-5p mimic. ** P < 0.001 vs Bio-NC. ( D ) The expression level of LPAR5 was detected by qRT-PCR in THCA tissues and paired adjacent normal tissues. ( E ) Pearson correlation analysis of the correlation between LPAR5 and miR-513c-5p in THCA tissues. ( F ) The expression level of LPAR5 was measured by qRT-PCR in the human thyroid follicular epithelial cell line (Nthy-ori 3-1) and THCA cell lines (TPC-1 and SW579). ** P < 0.001 vs Nthy-ori 3-1. ( G ) The mRNA expression level of LPAR5 were measured by Western blot in SW579 and TPC-1 cells transfected with miR-513c-5p mimic or miR-513c-5p inhibitor. Inhibitor, miR-513c-5p inhibitor. Mimic, miR-513c-5p mimic. ( H ) The protein expression level of LPAR5 were measured by Western blot in SW579 and TPC-1 cells transfected with miR-513c-5p mimic or miR-513c-5p inhibitor. Mimic, miR-513c-5p mimic. Inhibitor, miR-513c-5p inhibitor. Mimic, miR-513c-5p mimic. ** P < 0.001 vs Mimic-NC; ## P < 0.001 vs Inhibitor-NC. Abbreviations : Bio-NC, Biotin labeled negative contro
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8 MiR-513c-5p inhibits proliferation of THCA cells through LPAR5. ( A ) The protein expression level of LPAR5 were measured by Western blot in SW579 and TPC-1 cells transfected with si-LPARS or miR-513c-5p inhibitor. ( B ) Viability of SW579 and TPC-1 cells with si-LPARS or miR-513c-5p inhibitor transfection were detected by CCK-8 assay. ( C ) Proliferation of SW579 and TPC-1 cells with si-LPARS or miR-513c-5p inhibitor transfection were detected by BrdU assay. si-LPARS, silencing LPARS. Inhibitor, miR-513c-5p inhibitor. NC, co-transfected with si-LPARS-NC and inhibitor-NC. ** P < 0.001 vs blank; ## P < 0.001 vs si-LPAR5+inhibitor.