MA5-27774

antibody from Invitrogen Antibodies

Targeting: PPY PNP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27774

Provider

Invitrogen Antibodies

Product name

PPY Monoclonal Antibody (GT6512)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: human PPY-transfected 293T cells

Antibody clone number

GT6512

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PPY in A) 293T whole cell lysate, B) whole cell lysate of human PPY-transfected 293T cells using 30 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with PPY monoclonal antibody (Product # MA5-27774) using a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PPY Monoclonal Antibody (GT6512) detects PPY protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human PPY-transfected 293T cells.15 % SDS-PAGE. PPY Monoclonal Antibody (GT6512) (Product # MA5-27774) dilution: 1:5,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PPY was performed in Mock and transfected 293T cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PPY Monoclonal Antibody (GT6512) (Product # MA5-27774) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of PPY was performed in paraffin-embedded mouse pancreas tissue using PPY Monoclonal Antibody (GT6512) (Product # MA5-27774) at a dilution of 1:500.