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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PA5-98125 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTBP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.14 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of precipitated PTBP3 from A549 whole cell lysate using a PTBP3 polyclonal antibody (Product # PA5-98125). An HRP-conjugated Protein G antibody was used as the secondary antibody (1:50,000). Lane 1: Rabbit control IgG. Lane 2: A549 whole cell lysate (0.5mg). Lane 3: A549 whole cell lysate (20µg).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of precipitated PTBP3 from A549 whole cell lysate using a PTBP3 polyclonal antibody (Product # PA5-98125). An HRP-conjugated Protein G antibody was used as the secondary antibody (1:50,000). Lane 1: Rabbit control IgG. Lane 2: A549 whole cell lysate (0.5mg). Lane 3: A549 whole cell lysate (20µg).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PTBP3 using a PTBP3 Polyclonal antibody (Product # PA5-98125). Positive WB detected in: K562 whole cell lysate, MCF-7 whole cell lysate. All lanes: PTBP3 antibody at 1:2000. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 60 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PTBP3 in paraffin embedded human kidney tissue using a PTBP3 polyclonal antibody (Product # PA5-98125) at a dilution of 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
