Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-2273 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2273, RRID:AB_2096082
- Product name
- Rabbit Polyclonal EDC4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Poliovirus-mediated disruption of cytoplasmic processing bodies.
Junin virus infection impairs stress-granule formation in Vero cells treated with arsenite via inhibition of eIF2α phosphorylation.
Dougherty JD, White JP, Lloyd RE
Journal of virology 2011 Jan;85(1):64-75
Journal of virology 2011 Jan;85(1):64-75
Junin virus infection impairs stress-granule formation in Vero cells treated with arsenite via inhibition of eIF2α phosphorylation.
Linero FN, Thomas MG, Boccaccio GL, Scolaro LA
The Journal of general virology 2011 Dec;92(Pt 12):2889-2899
The Journal of general virology 2011 Dec;92(Pt 12):2889-2899
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EDC4 Antibody [NB100-2273] - Detection of Human and Mouse RCD8 on HeLa whole cell lysate using NB100-2273. RCD8 was also immunoprecipitated by another rabbit anti-RCD8 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EDC4 Antibody [NB100-2273] - Detection of human RCD8 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCD8 antibody NB100-2273 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EDC4 Antibody [NB100-2273] - Detection of mouse RCD8 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCD8 antibody NB100-2273 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: EDC4 Antibody [NB100-2273] - Detection of human RCD8 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RCD8 antibody NB100-2273 (lot NB100-2273-2) used for IP at 6 ug per reaction. RCD8 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2273-1). For blotting immunoprecipitated RCD8, A300-745A was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.