21316-1-AP

antibody from Proteintech Group

Targeting: C5AR1 C5A, C5AR, C5R1, CD88

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

21316-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#21316-1-AP, RRID:AB_10733105

Product name

C5aR antibody

Antibody type

Polyclonal

Description

C5aR antibody (Cat. #21316-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Akkermansia muciniphila Alleviates Porphyromonas gingivalis-induced Periodontal Disease by Enhancing Bacterial Clearance.

Hu Q, Leung WK, Acharya A, Li X, Pelekos G
Probiotics and antimicrobial proteins 2026 Jan;18(1):666-680

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Inhibiting Liver-Derived C3 Protein Rescues Anesthesia/Surgery-Induced Cognitive Impairment, Synaptic Disorders, and Microglial Phagocytosis.

Wu Q, Cong P, Li Z, Zhang Y, Wu H, Zhang Q, Li Y, Tian L, Miao Q, Zheng Y, Zhang H, Chen Q, Feng E, Li X, Chen Z, Wang DX, Huang X, Xiong L
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Jan;13(2):e02034

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Citalopram exhibits immune-dependent anti-tumor effects by modulating C5aR1(+) TAMs.

Dong F, Zhang S, Song K, Jiang L, Hu LP, Li Q, Zhang XL, Li J, Feng M, Cai ZW, Yao HF, Li RK, Li H, Chen J, Hu X, Wang J, Jiang C, Zhu HH, Wang C, Da LT, Zhang ZG, Bao Z, Wang X, Jiang SH
eLife 2026 Feb 9;14

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Treatment of Caries-Induced Pulpal Injury Using a Dynasore-Loaded Bonding System via Regulation of C5a/C5aR Endocytosis.

Chen Y, Han Z, Zhang Y, Zhai D, Yang W, Zheng S, Chen Z, Wang T, Liu C, Liu M
ACS applied bio materials 2026 Apr 20;9(8):3887-3905

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Sex-Specific Associations Between Cadmium Exposure and Mortality Risk in Cardiovascular Disease Patients: A Cohort Study Integrating Molecular Mechanisms of Myocardial Dysfunction.

He C, Wang K, Yang R, Li X, Shao Z, Ma L, Lu Y, Niu X, Zhang S, Wang Y
Cardiovascular toxicology 2026 Apr 17;26(5)

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KLHDC8A knockdown in normal ovarian epithelial cells promoted the polarization of pro-tumoral macrophages via the C5a/C5aR/p65 NFκB signaling pathway.

Fang J, Wang J, Zhao X, Yang Y, Xiao Y
Cellular immunology 2025 Mar-Apr;409-410:104913

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Regulation of phosphatase and tensin homolog by complement component 5a (C5a) and its receptor (C5aR1) in lupus nephritis: A novel therapeutic target.

Ma Y, Wang Y, Zhao P, Cheng L, Li L, Li R, Zhou X
Journal of cell communication and signaling 2025 Dec;19(4):e70055

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The molecular adjuvant effect of the C-terminal peptide of complement C5a in a teleost fish.

Liu B, Liang JX, Ma ZY, Zhong YQ, Sun Y, Wang XY, Zhang DF, Zhang YA, Zhang XJ
Fish & shellfish immunology 2025 Aug;163:110415

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Integrative proteogenomic characterization reveals therapeutic targets in poorly differentiated and anaplastic thyroid cancers.

Pan Z, Tan Z, Xu N, Yao Z, Zheng C, Shang J, Xie L, Xu J, Wang J, Jiang L, Zhu X, Yu D, Li Y, Che Y, Gong Y, Qin Z, Zhang Y, Zou X, Xu T, Guo Z, Jin T, Guo T, Wang W, Chen W, Sun Y, Wang W, Peng X, Yin C, Ding C, Huang P, Ge M
Nature communications 2025 Apr 16;16(1):3601

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Single-Cell RNA Sequencing Reveals That C5AR1 in Follicle Monocyte Cells Could Predict the Development of POI.

Han Y, Diao J, Wang X, Zhang S, Yuan L, Ping Y, Zhang Y, Luo H
Journal of inflammation research 2024;17:11221-11234

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The role of the C5a-C5aR pathway in iron metabolism and gastric cancer progression.

Ni Q, Yang H, Rao H, Zhang L, Xiong M, Han X, Deng B, Wang L, Chen J, Shi Y
Frontiers in immunology 2024;15:1522181

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A novel hollow iron nanoparticle system loading PEG-Fe(3)O(4) with C5a receptor antagonist for breast cancer treatment.

Yang H, Li G, Zhang J, Zhao J, Zhao Y, Wu Y, Sun Z, Song S, Zou Y, Zou Z, Han X, Deng B, Wang L, Rao H, Xu G, Wang S, Guo S, Ding H, Shi Y, Wu Y, Chen J
Frontiers in immunology 2024;15:1466180

---

Intracellular C5aR1 inhibits ferroptosis in glioblastoma through METTL3-dependent m6A methylation of GPX4.

Meng X, Wang Z, Yang Q, Liu Y, Gao Y, Chen H, Li A, Li R, Wang J, Sun G
Cell death & disease 2024 Oct 5;15(10):729

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C3a Mediates Endothelial Barrier Disruption in Brain-Derived, but Not Retinal, Human Endothelial Cells.

Wolf HN, Guempelein L, Schikora J, Pauly D
International journal of molecular sciences 2024 Oct 19;25(20)

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The role of C5aR1-mediated hepatic macrophage efferocytosis in NASH.

Shen X, Zheng W, Du X, Chen Y, Song X, Yang L, Yuan Q
Scientific reports 2024 Jul 26;14(1):17232

---

The C5aR1 complement receptor: A novel immunomodulator of insulin action in skeletal muscle.

Shah DS, McNeilly AD, McCrimmon RJ, Hundal HS
Cellular signalling 2024 Jan;113:110944

---

Blockade of C5a receptor unleashes tumor-associated macrophage antitumor response and enhances CXCL9-dependent CD8(+) T cell activity.

Luan X, Lei T, Fang J, Liu X, Fu H, Li Y, Chu W, Jiang P, Tong C, Qi H, Fu Y
Molecular therapy : the journal of the American Society of Gene Therapy 2024 Feb 7;32(2):469-489

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Identification of a novel macrophage-related prognostic signature in colorectal cancer.

Lin D, Zheng T, Huang S, Liu R, Guan S, Zhang Z
Scientific reports 2024 Feb 2;14(1):2767

---

C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways.

Chen Y, Liu Y, Li H, Huna R, Tan X, Li N, Zhang Y, Jiao X, Liu M
Journal of applied oral science : revista FOB 2023;31:e20220404

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LPS-induced inflammation potentiates dental pulp stem cell odontogenic differentiation through C5aR and p38.

Kim JH, Irfan M, Hossain MA, Shin S, George A, Chung S
Connective tissue research 2023 Sep;64(5):505-515

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Determination of the optimal concentration and duration of C5aR antagonist application in an inflammatory model of human dental pulp cells.

Hu J, Tan X, Wei X, Hu W, Gao L, Cao X, Yang H, Jiang Z, Li N, Teng L, Liu M
FEBS open bio 2023 Mar;13(3):570-581

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C5aR1 promotes the progression of colorectal cancer by EMT and activating Wnt/β-catenin pathway.

Xu D, Li M, Ran L, Li X, Sun X, Yin T
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 2023 Feb;25(2):440-446

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Vitamin D/Vitamin D receptor mitigates cisplatin-induced acute kidney injury by down-regulating C5aR.

Wen Z, Sun C, Lou Y, Kong J
Journal of immunotoxicology 2023 Dec;20(1):2248267

---

Suppressing MDSC Infiltration in Tumor Microenvironment Serves as an Option for Treating Ovarian Cancer Metastasis.

Li Y, Zhang Q, Wu M, Zhang P, Huang L, Ai X, Yang Z, Shen Q, Wang Y, Wang P, Zhou S, He ML
International journal of biological sciences 2022;18(9):3697-3713

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QiShenYiQi Pill Ameliorates Cardiac Fibrosis After Pressure Overload-Induced Cardiac Hypertrophy by Regulating FHL2 and the Macrophage RP S19/TGF-β1 Signaling Pathway.

Anwaier G, Xie TT, Pan CS, Li AQ, Yan L, Wang D, Chen FK, Weng DZ, Sun K, Chang X, Fan JY, Han JY, Liu J
Frontiers in pharmacology 2022;13:918335

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Inhibition of the immunoproteasome modulates innate immunity to ameliorate muscle pathology of dysferlin-deficient BlAJ mice.

Farini A, Tripodi L, Villa C, Napolitano F, Strati F, Molinaro D, Facciotti F, Cassani B, Torrente Y
Cell death & disease 2022 Nov 19;13(11):975

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HSP90-Mediates Liraglutide Preconditioning-Induced Cardioprotection by Inhibiting C5a and NF-κB.

He ST, Wang DX, Meng JJ, Cheng XF, Bi Q, Zhong GQ, Tu RH
Journal of investigative surgery : the official journal of the Academy of Surgical Research 2022 May;35(5):1012-1020

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C5a complement receptor modulates odontogenic dental pulp stem cell differentiation under hypoxia.

Pasiewicz R, Valverde Y, Narayanan R, Kim JH, Irfan M, Lee NS, George A, Cooper LF, Alapati SB, Chung S
Connective tissue research 2022 Jul;63(4):339-348

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The role of complement C5a receptor in DPSC odontoblastic differentiation and in vivo reparative dentin formation.

Irfan M, Kim JH, Marzban H, Reed DA, George A, Cooper LF, Chung S
International journal of oral science 2022 Jan 27;14(1):7

---

Complement C5aR/LPS-induced BDNF and NGF modulation in human dental pulp stem cells.

Irfan M, Kim JH, Druzinsky RE, Ravindran S, Chung S
Scientific reports 2022 Feb 7;12(1):2042

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Activation of C3 and C5 May Be Involved in the Inflammatory Progression of PCM and GM.

Li XQ, Sun HG, Wang XH, Zhang HJ, Zhang XS, Yu Y, Liu J, Guo QQ, Yang ZL
Inflammation 2022 Apr;45(2):739-752

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Linking Complement C3 and B Cells in Nasal Polyposis.

Werner U, Künstner A, Drenckhan M, Pries R, Bruchhage KL, Busch HS, Bachert C, Wollenberg B
Journal of immunology research 2020;2020:4832189

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Cerebral Immunohistochemical Characterization of the H(2)S and the Oxytocin Systems in a Porcine Model of Acute Subdural Hematoma.

Denoix N, Merz T, Unmuth S, Hoffmann A, Nespoli E, Scheuerle A, Huber-Lang M, Gündel H, Waller C, Radermacher P, McCook O
Frontiers in neurology 2020;11:649

---

Complement Activation and Organ Damage After Trauma-Differential Immune Response Based on Surgical Treatment Strategy.

Lackner I, Weber B, Baur M, Fois G, Gebhard F, Pfeifer R, Cinelli P, Halvachizadeh S, Lipiski M, Cesarovic N, Schrezenmeier H, Huber-Lang M, Pape HC, Kalbitz M
Frontiers in immunology 2020;11:64

---

The hepatocyte-specifically expressed lnc-HSER alleviates hepatic fibrosis by inhibiting hepatocyte apoptosis and epithelial-mesenchymal transition.

Zhang K, Zhang M, Yao Q, Han X, Zhao Y, Zheng L, Li G, Liu Q, Chang Y, Zhang P, Cui H, Shi Z, Chen T, Yao Z, Han T, Hong W
Theranostics 2019;9(25):7566-7582

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N-Acetylcysteine Attenuates Cisplatin-Induced Acute Kidney Injury by Inhibiting the C5a Receptor.

Huang S, You J, Wang K, Li Y, Zhang Y, Wei H, Liang X, Liu Y
BioMed research international 2019;2019:4805853

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Lung injury after asphyxia and hemorrhagic shock in newborn piglets: Analysis of structural and inflammatory changes.

Weber B, Mendler MR, Lackner I, von Zelewski A, Höfler S, Baur M, Braun CK, Hummler H, Schwarz S, Pressmar J, Kalbitz M
PloS one 2019;14(7):e0219211

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Time-dependent C5a and C5aR expression in dental pulp cells following stimulation with LTA and LPS.

Liu M, Mu H, Peng W, Zhao L, Hu W, Jiang Z, Gao L, Cao X, Li N, Han J
International journal of molecular medicine 2019 Sep;44(3):823-834

---

RNA sequencing screening of differentially expressed genes after spinal cord injury.

Li Y, Chen Y, Li X, Wu J, Pan JY, Cai RX, Yang RY, Wang XD
Neural regeneration research 2019 Sep;14(9):1583-1593

---

Diet-Induced Obesity Affects Muscle Regeneration After Murine Blunt Muscle Trauma-A Broad Spectrum Analysis.

Xu P, Werner JU, Milerski S, Hamp CM, Kuzenko T, Jähnert M, Gottmann P, de Roy L, Warnecke D, Abaei A, Palmer A, Huber-Lang M, Dürselen L, Rasche V, Schürmann A, Wabitsch M, Knippschild U
Frontiers in physiology 2018;9:674

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Experimental blunt chest trauma-induced myocardial inflammation and alteration of gap-junction protein connexin 43.

Kalbitz M, Amann EM, Bosch B, Palmer A, Schultze A, Pressmar J, Weber B, Wepler M, Gebhard F, Schrezenmeier H, Brenner R, Huber-Lang M
PloS one 2017;12(11):e0187270

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Cardiac Depression in Pigs after Multiple Trauma - Characterization of Posttraumatic Structural and Functional Alterations.

Kalbitz M, Schwarz S, Weber B, Bosch B, Pressmar J, Hoenes FM, Braun CK, Horst K, Simon TP, Pfeifer R, Störmann P, Hummler H, Gebhard F, Pape HC, Huber-Lang M, Hildebrand F, TREAT Research Group
Scientific reports 2017 Dec 19;7(1):17861

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Nerve Growth Factor Secretion From Pulp Fibroblasts is Modulated by Complement C5a Receptor and Implied in Neurite Outgrowth.

Chmilewsky F, Ayaz W, Appiah J, About I, Chung SH
Scientific reports 2016 Aug 19;6:31799

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Complement is a central mediator of radiotherapy-induced tumor-specific immunity and clinical response.

Surace L, Lysenko V, Fontana AO, Cecconi V, Janssen H, Bicvic A, Okoniewski M, Pruschy M, Dummer R, Neefjes J, Knuth A, Gupta A, van den Broek M
Immunity 2015 Apr 21;42(4):767-77

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 21316-1-AP(C5AR1 antibody) at dilution of 1:800

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using C5AR1 antibody 21316-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 21316-1-AP(C5AR1 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 21316-1-AP(C5AR1 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue