H00000728-M02

antibody from Abnova Corporation

Targeting: C5AR1 C5A, C5AR, C5R1, CD88

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000728-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000728-M02, RRID:AB_1572362

Product name

C5R1 monoclonal antibody (M02), clone 4E2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant C5R1.

Antigen sequence

MNSFNYTTPDYGHYDDKDTLDLNTPVDKTSNTLRV
PDILALVIFAVVFLVGVLGNALVVWVTAFEAKRTI
NAIWFLNLAVADFLSCLALPILFTSIVQHHHWPFG
GAACSILPSLILLNMYASILLLATISADRFLLVFK
PIWCQNFRGAGLAWIACAVAWGLALLLTIPSFLYR
VVREEYFPPKVLCGVDYSHDKRRERAVAIVRLVLG
FLWPLLTLTICYTFILLRTWSRRATRSTKTLKVVV
AVVASFFIFWLPYQVTGIMMSFLEPSSPTFLLLNK
LDSLCVSFAYINCCINPIIYVVAGQGFQGRLRKSL
PSLLRNVLTEESVVRESKSFTRSTVDTMAQKTQAV


Isotype

IgG

Antibody clone number

4E2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of C5R1 expression in transfected 293T cell line by C5R1 monoclonal antibody (M02), clone 4E2.Lane 1: C5R1 transfected lysate(39.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged C5AR1 is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol