H00000728-D01P

antibody from Abnova Corporation

Targeting: C5AR1 C5A, C5AR, C5R1, CD88

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000728-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000728-D01P, RRID:AB_1673189

Product name

C5AR1 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human C5AR1 protein.

Antigen sequence

MNSFNYTTPDYGHYDDKDTLDLNTPVDKTSNTLRV
PDILALVIFAVVFLVGVLGNALVVWVTAFEAKRTI
NAIWFLNLAVADFLSCLALPILFTSIVQHHHWPFG
GAACSILPSLILLNMYASILLLATISADRFLLVFK
PIWCQNFRGAGLAWIACAVAWGLALLLTIPSFLYR
VVREEYFPPKVLCGVDYSHDKRRERAVAIVRLVLG
FLWPLLTLTICYTFILLRTWSRRATRSTKTLKVVV
AVVASFFIFWLPYQVTGIMMSFLEPSSPTFLLLNK
LDSLCVSFAYINCCINPIIYVVAGQGFQGRLRKSL
PSLLRNVLTEESVVRESKSFTRSTVDTMAQKTQAV


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of C5AR1 expression in transfected 293T cell line (H00000728-T02) by C5AR1 MaxPab polyclonal antibody.Lane 1: C5AR1 transfected lysate(39.30 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between C5AR1 and GNAI2. HeLa cells were stained with anti-C5AR1 rabbit purified polyclonal 1:1200 and anti-GNAI2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)