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antibody from Invitrogen Antibodies
Targeting: CCR2
CC-CKR-2, CD192, CKR2, CMKBR2, FLJ78302, MCP-1-R
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Flow cytometry [3]
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- Product number
- PA5-23037 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCR2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The immunogen sequence similarity with other species: Rhesus monkey and several other primate species (100%), Mouse (69%).
- Reactivity
- Human, Mouse, Rat, Bovine, Feline
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references Nephrogenic Systemic Fibrosis Is Mediated by Myeloid C-C Chemokine Receptor 2.
Gadolinium-based contrast agents: Stimulators of myeloid-induced renal fibrosis and major metabolic disruptors.
In renal transplants with delayed graft function chemokines and chemokine receptor expression predict long-term allograft function.
Do C, Drel V, Tan C, Lee D, Wagner B
The Journal of investigative dermatology 2019 Oct;139(10):2134-2143.e2
The Journal of investigative dermatology 2019 Oct;139(10):2134-2143.e2
Gadolinium-based contrast agents: Stimulators of myeloid-induced renal fibrosis and major metabolic disruptors.
Do C, Ford B, Lee DY, Tan C, Escobar P, Wagner B
Toxicology and applied pharmacology 2019 Jul 15;375:32-45
Toxicology and applied pharmacology 2019 Jul 15;375:32-45
In renal transplants with delayed graft function chemokines and chemokine receptor expression predict long-term allograft function.
Dikow R, Becker LE, Schaier M, Waldherr R, Gross ML, Zeier M
Transplantation 2010 Oct 15;90(7):771-6
Transplantation 2010 Oct 15;90(7):771-6
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CCR2 using a polyclonal antibody (Product # PA5-23037).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CCR2 in formalin-fixed paraffin-embedded tissue section of human liver cancer. Samples were incubated in CCR2 polyclonal antibody (Product # PA5-23037) using a dilution of 5 µg/mL. Intense cytoplasmic-membranous immune-staining was observed in the cancerous hepatocytes, whereas the staining was relatively weak in the cells adjacent to the fibrotic areas. [Magnification 40X].
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CCR2 in formalin-fixed paraffin-embedded tissue section of human normal breast. Samples were incubated in CCR2 polyclonal antibody (Product # PA5-23037) using a dilution of 5 µg/mL. The breast ductal/acinar epithelial cells showed a strong cytoplasmic-membranous CCR2 immune-positivity, whereas the intra-lobular connective tissue depicted weak staining. [Magnification 40X].
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CCR2 in formalin-fixed paraffin-embedded section of human esophageal squamous cell carcinoma (SCC). Samples were incubated in CCR2 polyclonal antibody (Product # PA5-23037) using a dilution of 5 µg/mL. Strong cytoplasmic-membranous immune-staining was observed in SCC cells and a relatively weaker staining was seen in the tumor stroma cells. [Magnification 10X].
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CCR2 in formalin-fixed paraffin-embedded tissue section of human rectal adenocarcinoma. Samples were incubated in CCR2 polyclonal antibody (Product # PA5-23037) using a dilution of 5 µg/mL. The cancerous cells in the section developed a uniform but specific CCR2 immunopositivity whereas a weak staining was seen in the tumor stroma cells. [Magnification 40X].
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of CCR2 in formalin-fixed paraffin-embedded tissue section of human normal kidney. Samples were incubated in CCR2 polyclonal antibody (Product # PA5-23037) using a dilution of 5 µg/mL. Strong cytoplasmic immunostaining was observed in the various renal cells (especially from tubules/ducts epithelium). The cells of Bowmans capsule depicted a very weak cytoplasmic staining for CCR2 protein. [Magnification 10X].
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CCR2 using a polyclonal antibody (Product # PA5-23037).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CCR2 in THP-1 cells. Samples were incubated in CCR2 polyclonal antibody (Product # PA5-23037) using a dilution of 5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to DyLight 550.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CCR2 in THP-1 cells. Samples were incubated in CCR2 polyclonal antibody (Product # PA5-23037) using a dilution of 5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to DyLight 550.
