Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 13571-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARFGAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MASPRTRKV LKEVRVQDEN NVCFECGAFN PQWVSVTYGI WICLECSGRH RGLGVHLSFV RSVTMDKWKD IELEKMKAGG NAKFREFLES QEDYDPCWSL QEKYNSRAAA LFRDKVVALA EGREWSLESS PAQNWTPPQP RTLPSMVHRV SGQPQSVTAS SDKAFEDWLN DDLGSYQGAQ GNRYVGFGNT PPPQKKEDDF LNNAMSSLYS GWSSFTTGAS RFASAAKEGA TKFGSQASQK FWGHKQQPEP ASELGHSLNE NVLKPAQEKV KEGKIFDDVS SGVSQLASKG VGSKGWRDVT TFFSGKAEGP LDSPSEGHSY QNSGLDHFQN SNIDQSFWET FGSAEPTKTR KSPSSDSWTC ADTSTERRSS DSWEVWGSAS TNRNSNSDGG EGGEGTKKAV PPAVPTDDGW DNQNW (1-414 aa encoded by BC028233)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.41 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 13571-1-AP (ARFGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 13571-1-AP (ARFGAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 13571-1-AP (ARFGAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed PC-3 cells using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 13571-1-AP) (ARFGAP1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed PC-3 cells using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer using 13571-1-AP (ARFGAP1 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ARFGAP1 (IP:13571-1-AP, 4ug; Detection:13571-1-AP 1:500) with mouse testis tissue lysate 4000ug.