NBP2-00513

antibody from Novus Biologicals

Targeting: ARFGAP1 ARF1GAP, bA261N11.3, FLJ10767

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-00513

Provider

Novus Biologicals

Product name

Mouse Monoclonal ARFGAP1 Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human, Simian

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

0.63 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ARFGAP1 Antibody (1F6) [NBP2-00513] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARFGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARFGAP1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ARFGAP1 Antibody (1F6) [NBP2-00513] Analysis of extracts (35ug) from 9 different cell lines by using anti-ARFGAP1 monoclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ARFGAP1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ARFGAP1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ARFGAP1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Human endometrium tissue using anti-ARFGAP1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Human Kidney tissue using anti-ARFGAP1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Human liver tissue using anti-ARFGAP1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Human lymphoma tissue using anti-ARFGAP1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Human pancreas tissue using anti-ARFGAP1 mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: ARFGAP1 Antibody (1F6) [NBP2-00513] - Staining of paraffin-embedded Human thyroid tissue using anti-ARFGAP1 mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: ARFGAP1 Antibody (1F6) [NBP2-00513] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARFGAP1 antibody, and then analyzed by flow cytometry.