HPA045156

antibody from Atlas Antibodies

Targeting: POLR2I hRPB14.5, RPB9

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA045156

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA045156, RRID:AB_10964748

Product name

Anti-POLR2I

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human POLR2I, Gene description: polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa, Alternative Gene Names: hRPB14.5, RPB9, Validated applications: IHC, ICC, WB, ChIP, Uniprot ID: P36954, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

ChIP-Exo-Seq composite graph for Anti-POLR2I (HPA045156, Lot 000026049) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.