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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA328607 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal Anti-Nicotinic Acetylcholine Receptor alpha 4 (extracellular
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal Anti-Nicotinic Acetylcholine Receptor alpha 4 (extracellular
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- CHRNA4
- Antibody clone number
- NULL
- Vial size
- 200 µl
- Concentration
- NULL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain cell lysates: 1, 2. Anti-Nicotinic Acetylcholine Receptor a4 (extracellular) antibody, (1:200). 3, 4. Anti-Nicotinic Acetylcholine Receptor a4 (extracellular) antibody, preincubated with the control peptide antigen.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- pression of nAChaa4 in rat cerebellum. Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nicotinic Acetylcholine Receptor a4 (extracellular) antibody, (1:200). A. Expression of nAChRa4 (green) was visualized in rat cerebellum. Purkinje cells were positive for nAChRa4 (arrows). B. The same section was stained for Parvalbumin (red) and counterstained (blue).
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Indirect flow cytometry analysis in live intact Jurkat (human T cell leukemia) cell line: black line, Unstained cells + goat-anti-rabbit-Cy5. green line, Cells + Anti-Nicotinic Acetylcholine Receptor a4 (extracellular) antibody, (1:20) + goat-anti-rabbit-Cy5.
- Validation comment
- FC
