Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-77502 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHRNA4 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain cell lysates with CHRNA4 (extracellular) polyclonal antibody (Product # PA5-77502) using a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain cell lysates with CHRNA4 (extracellular) polyclonal antibody (Product # PA5-77502) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CHRNA4 (extracellular) in perfusion-fixed, frozen mouse brain. A) Samples were probed with CHRNA4 (extracellular) polyclonal antibody (Product # PA5-77502) using a dilution of 1:300 (green), and incubated with fluorescent 594 and DAPI. B) Parietal cortex stained image (red). C) Merged image of Panels A and B.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of CHRNA4 (extracellular) in perfusion-fixed, frozen mouse brain. A) Samples were probed with CHRNA4 (extracellular) polyclonal antibody (Product # PA5-77502) using a dilution of 1:300 (green), and incubated with fluorescent 594 and DAPI. B) Parietal cortex stained image (red). C) Merged image of Panels A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Jurkat cells using polyclonal CHRNA4 (extracellular) antibody (Product # PA5-77502) with a dilution of 1:20 followed by a goat-anti-rabbit-Cy5 secondary antibody.