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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-29791 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHRNA4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
- Concentration
- 0.39 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nicotinic Acetylcholine Receptor alpha 4 using 30 µg of A) 293T (B) H1299 (C) HepG2 and D) MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Nicotinic Acetylcholine Receptor alpha 4 polyclonal antibody (Product # PA5-29791) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CHRNA4 Polyclonal Antibody (Product # PA5-29791). Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CHRNA4 Polyclonal Antibody (Product # PA5-29791) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft , using Nicotinic Acetylcholine Receptor alpha 4 (Product # PA5-29791) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
