Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-87987 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87987, RRID:AB_11005965
- Product name
- Rabbit Polyclonal Testin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Testin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references RNA deep sequencing as a tool for selection of cell lines for systematic subcellular localization of all human proteins.
Danielsson F, Wiking M, Mahdessian D, Skogs M, Ait Blal H, Hjelmare M, Stadler C, Uhlén M, Lundberg E
Journal of proteome research 2013 Jan 4;12(1):299-307
Journal of proteome research 2013 Jan 4;12(1):299-307
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Testin Antibody [NBP1-87987] - Simple Western lane view shows a specific band for TES in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Testin Antibody [NBP1-87987] - Electropherogram image(s) of corresponding Simple Western lane view. Testin antibody was used at 1:20 dilution on RT-4 lysate(s).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Testin Antibody [NBP1-87987] - Analysis in human cell line EFO-21.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Testin Antibody [NBP1-87987] - Analysis in human cell lines A-431 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Testin Antibody [NBP1-87987] - Analysis using Anti-TES antibody NBP1-87987 (A) shows similar pattern to independent antibody NBP1-85072 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human tonsil shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human endometrium moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human lymphoid tissues shows moderate cytoplasmic positivity in non-germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human endometrium moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human liver shows no cytoplasmic positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Testin Antibody [NBP1-87987] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.