Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunohistochemistry [6]
- Other assay [1]
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Validation data
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- Product number
- 22709-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EML4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: GSLDDSISA ASTSDVQDRL SALESRVQQQ EDEITVLKAA LADVLRRLAI SEDHVASVKK SVSSKGQPSP RAVIPMSCIT NGSGANRKPS HTSAVSIAGK ETLSSAAKSG TEKKKEKPQG QREKKEESHS NDQSPQIRAS PSPQPSSQPL QIHRQTPESK NATPTKSIKR PSPAEKSHNS WENSDDSRNK LSKIPSTPKL IPKVTKTADK HKDVIINQEG EYIKMFMRGR PITMFIPSDV DNYDDIRTEL PPEKLKLEWA YGYRGKDCRA NVYLLPTGEI VYFIASVVVL FNYEERTQRH YLGHTDCVKC LAIHPDKIRI ATGQIAGVDK DGRPLQPHVR VWDSVTLSTL QIIGLGTFER GVGCLDFSKA DSGVHLCVID DSNEHMLTVW DWQKKAKGAE IKTTNEVVLA VEFHPTDANT IITCGKSHIF FWTWSGNSLT RKQGIFGKYE KPKFVQCLAF LGNGDVLTGD SGGVMLIWSK TTVEPTPGKG PKGVYQISKQ IKAHDGSVFT LCQMRNGMLL TGGGKDRKII LWDHDLNPER EIEVPDQYGT IRAVAEGKAD QFLVGTSRNF ILRGTFNDGF QIEVQGHTDE LWGLATHPFK DLLLTCAQDR QVCLWNSMEH RLEWTRLVDE PGHCADFHPS GTVVAIGTHS GRWFVLDAE (6-663 aa encoded by BC140845 )
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.44 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 22709-1-AP (EML4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 22709-1-AP (EML4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 22709-1-AP (EML4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A549 cells using 22709-1-AP (EML4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A549 cells using 22709-1-AP (EML4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 22709-1-AP (EML4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 22709-1-AP ( EML4 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 22709-1-AP ( EML4 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-EML4 (IP:22709-1-AP, 4ug; Detection:22709-1-AP 1:2000) with A549 cells lysate 400ug.