PA5-114001

antibody from Invitrogen Antibodies

Targeting: ZCCHC12 FLJ16123, PNMA7A, SIZN, SIZN1

Western blot

Antibody data

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Validation data

Product number: PA5-114001 - Provider product page
Provider: Invitrogen Antibodies
Product name: ZCCHC12 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Immunogen sequence: EKASLYVIRL EVQLQNAIQA GIIAEKDANR TRLQQLLLGG ELSRDLRLRL For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. Predicted homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.5 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

ZCCHC12 protein structure - PA5-114001 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 ZCCHC12 is a target gene of miR-376c-3p in PTC cells. (a) Expression levels of candidate miRNAs (miR-376c-3p and miR-325-3p) in thyroid epithelial cell line and PTC cell lines were examined using RT-qPCR. (b) RT-qPCR was performed to detect miR-376c-3p level in PTC cells with transfection of miR-376c-3p mimics. (c) The binding site between miR-376c-3p and ZCCHC12 was searched on the TargetScan website. (d and e) Luciferase reporter assays and RNA pulldown assays were conducted to testify the interaction between miR-376c-3p and ZCCHC12. (f) The influence of miR-376c-3p mimics on mRNA and protein levels of ZCCHC12 were evaluated by RT-qPCR and western blot. * p < 0.05, ** p < 0.01, *** p < 0.001.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 LINC00475 upregulates ZCCHC12 expression by binding to miR-376c-3p. (a) lncRNAs that can bind to miR-376c-3p were predicted with the LncBase. (b) RT-qPCR was conducted to detect the LINC00475 level in PTC cells and normal thyroid epithelial cells. (c) Subcellular fractionation assays were performed to determine the distribution of LINC00475 in TPC1 and BCPAP cells. (d) After transfection with sh-LINC00475#1/2, RT-qPCR was used for the detection of LINC00475 expression in PTC cells. (e) The binding site between LINC00475 and miR-376c-3p was predicted by the LncBase. (f) Luciferase reporter assays were conducted to testify the binding between LINC00475 and miR-376c-3p. (g) The interaction between miR-376c-3p and LINC00475 (or ZCCHC12) was further evaluated by RIP assays. (h) RT-qPCR was utilized to detect miR-376c-3p expression in PTC cells after transfection of sh-LINC00475#2. (i and j) RT-qPCR and western blot were conducted to assess ZCCHC12 expression in PTC cells after transfection of sh-LINC00475#2. ** p < 0.01, *** p < 0.001.