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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- NBP1-77972 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77972, RRID:AB_11023706
- Product name
- Mouse Monoclonal ESRP2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. A BLAST analysis was used to suggest cross-reactivity with Esrp-2 from mouse based on a 100% identity with the immunizing sequence. Cross-reactivity with Esrp-2 from other sources has not been determined.
- Reactivity
- Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition.
Warzecha CC, Jiang P, Amirikian K, Dittmar KA, Lu H, Shen S, Guo W, Xing Y, Carstens RP
The EMBO journal 2010 Oct 6;29(19):3286-300
The EMBO journal 2010 Oct 6;29(19):3286-300
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ESRP2 Antibody (22C5.H7.A6) [NBP1-77972] - Anti-ESRP2 by Detection of ESRP2 in transfected 293T cell extracts (lane 2). Lanes G and 1 contain 5ug GFP-transfected- and ESRP1-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 ug of lysate. Primary antibody was used at a 1:1000 dilution (PBS-T plus milk) and reacted for O/N at 4C. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. The bands shown are full length FLAG-ESRP2 (~80kDa) and a slightly lower band that is specific to ESRP2.
