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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PA5-34891 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PI16 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116. Predicted reactivity: Mouse (81%), Rat (83%), Pig (84%), Rhesus Monkey (96%), Chimpanzee (99%), Bovine (87%). IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.86 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot using PI16 Polyclonal Antibody (Product # PA5-34891). Sample (30 µg of whole cell lysate). Lane A: A549. 10% SDS PAGE. PI16 Polyclonal Antibody (Product # PA5-34891) diluted at 1:1,000.
