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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Product number
- PA5-21821 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Bisphosphoglycerate mutase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, Molt-4, Raji, mouse spleen.
- Concentration
- 0.84 mg/mL
Submitted references Bisphosphoglycerate mutase controls serine pathway flux via 3-phosphoglycerate.
Oslund RC, Su X, Haugbro M, Kee JM, Esposito M, David Y, Wang B, Ge E, Perlman DH, Kang Y, Muir TW, Rabinowitz JD
Nature chemical biology 2017 Oct;13(10):1081-1087
Nature chemical biology 2017 Oct;13(10):1081-1087
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Bisphosphoglycerate mutase Polyclonal Antibody (Product # PA5-21821). Sample (30 µg whole cell lysate). A: 293T. B: A431. C: H1299. 12% SDS PAGE. Bisphosphoglycerate mutase Polyclonal Antibody (Product # PA5-21821) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Bisphosphoglycerate mutase Polyclonal Antibody (Product # PA5-21821). Sample (50 µg of whole cell lysate). Lane A: mouse spleen. 12% SDS PAGE. Bisphosphoglycerate mutase Polyclonal Antibody (Product # PA5-21821) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of bisphosphoglycerate mutase in paraformaldehyde-fixed HeLa cells using a bisphosphoglycerate mutase polyclonal antibody (Product # PA5-21821) at a 1:100 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BPGM (Product # PA5-21821) antibody at 1:100 dilution.
