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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-35404 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-EEF1A2 (Ser358) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with chicken, non-human primate, rat, sheep and Xenopus based on 100% sequence homology.
- Concentration
- ~0.15 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-EEF1A2 pSer358 in mouse hippocampal lysate using a Phospho-EEF1A2 pSer358 polyclonal antibody (Product # PA5-35404). Results show a band at ~50kDa. Immunolabeling is blocked by treatment of the lysate with lambda phosphatase (P-tase) (30 minutes, 800 units/1 mg protein).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of EEF1A2 in mouse hippocampal lysate showing specific immunolabeling of a band at ~50 kDa corresponding to Phospho-EEF1A2 (Ser358) polyclonal antibody (Product # PA5-35404) in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (800 units/1 mg protein for 30 min).
