PA5-117923

antibody from Invitrogen Antibodies

Targeting: EEF1A2 EEF1AL, HS1, STN, STNL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117923

Provider

Invitrogen Antibodies

Product name

EEF1A2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of EEF1A2 in Lane A: HepG2 Whole Cell Lysate, Lane B: MCF7 Whole Cell Lysate, Lane C: U-251 MG Whole Cell Lysate, Lane D: Jurkat Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-117923) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 50 kDa, Observed band size: 50 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using EEF1A2 Polyclonal Antibody (Product # PA5-117923) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: MCF7 Whole Cell Lysate, Lane C: U-251 MG Whole Cell Lysate, Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of EEF1A2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with EEF1A2 Polyclonal Antibody (Product # PA5-117923, 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of EEF1A2 in Lane A: 0.5 mg U-251MG Whole Cell Lysate. Samples were treated with 60 μg of immunomagnetic Protein A/G beads, incubated with polyclonal antibody (Product # PA5-117923) with a dilution of 1:100 , followed by Clean-Blot HRP Detection reagent using a dilution of 1:1,000. Assay was performed under reducing conditions. Predicted band size: 50 kDa , Observed band size : 50 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EEF1A2 Immunoprecipitation using: Lane A: 0.5 mg U-251MG Whole Cell Lysate 4 µL with EEF1A2 Polyclonal Antibody (Product # PA5-117923) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: EEF1A2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 50 kDa. Observed band size: 50 kDa.