PA5-27676

antibody from Invitrogen Antibodies

Targeting: EEF1A2 EEF1AL, HS1, STN, STNL

Antibody data

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Validation data

Product number: PA5-27676 - Provider product page
Provider: Invitrogen Antibodies
Product name: EEF1A2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain, mouse muscle, rat brain, EEF1A2 shRNA-transfected 293T. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (97%), Xenopus laevis (99%), Rabbit (100%), Chicken (98%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.21 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

EEF1A2 protein structure - PA5-27676 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of EEF1A2 using 30 µg of A) HeLa (B) HepG2 (C) MOLT4 and D) Raji lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an EEF1A2 polyclonal antibody (Product # PA5-27676) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of EEF1A2 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a EEF1A2 Polyclonal Antibody (Product # PA5-27676) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using EEF1A2 Polyclonal Antibody (Product # PA5-27676). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. EEF1A2 Polyclonal Antibody (Product # PA5-27676) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of EEF1A2 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a EEF1A2 Polyclonal Antibody (Product # PA5-27676) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: EEF1A2 Polyclonal Antibody detects EEF1A2 protein by western blot analysis. A. 50 µg mouse muscle lysate/extract.10% SDS-PAGE. EEF1A2 Polyclonal Antibody (Product # PA5-27676) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.