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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- PA5-27677 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EEF1A2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain, rat brain. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (95%), Xenopus laevis (98%), Rabbit (100%), Chicken (99%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EEF1A2 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a EEF1A2 Polyclonal Antibody (Product # PA5-27677) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EEF1A2 Polyclonal Antibody (Product # PA5-27677). Sample (30 µg whole cell lysate). A: 293T. B: A431. C: H1299. D: HeLa S3. E: HepG2. F: MOLT4. G: Raji. 10% SDS PAGE. EEF1A2 Polyclonal Antibody (Product # PA5-27677) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EEF1A2 Polyclonal Antibody (Product # PA5-27677). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. EEF1A2 Polyclonal Antibody (Product # PA5-27677) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EEF1A2 Polyclonal Antibody detects EEF1A2 protein at cytosol on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. EEF1A2 Polyclonal Antibody (Product # PA5-27677) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EEF1A2 Polyclonal Antibody detects EEF1A2 protein at cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. EEF1A2 Polyclonal Antibody (Product # PA5-27677) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
