MA1-830

antibody from Invitrogen Antibodies

Targeting: PLA2G5

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-830

Provider

Invitrogen Antibodies

Product name

PLA2G5 Monoclonal Antibody (3G1)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-830 contains 100 µg of in vitro produced, protein A purified antibody (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide. MA1-830 detects Group V Secretory Phospholipase A2 from human and mouse samples. MA1-830 has been successfully used in Western blot procedures. By Western blot, MA1-830 detects a ~15 kDa band representing Group V Secretory Phospholipase A2 from mouse macrophages. The MA1-830 immunogen is full length W79A human group V phospholipase A2.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

3G1

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Characterization of monoclonal antibodies specific for 14-kDa human group V secretory phospholipase A2 (hVPLA2).

Muñoz NM, Kim KP, Han SK, Boetticher E, Sperling AI, Sano H, Zhu X, Cho W, Leff AR
Hybridoma 2000 Apr;19(2):171-6

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Group V secretory phospholipase A2 (PLAG5) was performed using 90% confluent log phase NCCIT cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PLA2G5 (3G1) Mouse Monoclonal Antibody (Product # MA1-830) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.