22117-1-AP

antibody from Proteintech Group

Targeting: PPP1R12A M130, MBS, MYPT1

Provider product page for 22117-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [25]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 22117-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#22117-1-AP, RRID:AB_11183753
Product name: MYPT1 antibody
Antibody type: Polyclonal
Description: KD/KO validated MYPT1 antibody (Cat. #22117-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PPP1R12A protein structure - 22117-1-AP shown in red.

Submitted references NUAK1 silencing enhances radiotherapy-induced ferroptosis in locally advanced rectal cancer by impairing Nrf2-driven transcription of GPX4.

Zhuang Z, Guan B, Wang Y, Lin X, Cai H, Chen B, Li S, Liu X, Zhuang J, Guan G
Cellular oncology (Dordrecht, Netherlands) 2026 Mar 18;49(2)

A fetal oncogene NUAK2 is an emerging therapeutic target in glioblastoma.

Jo H, Munoz S, Dalvi A, Yang W, Morozova E, Glasgow SM
EMBO molecular medicine 2025 Sep;17(9):2409-2437

Non-canonical activation of MAPK signaling by the lncRNA ASH1L-AS1-encoded microprotein APPLE through inhibition of PP1/PP2A-mediated ERK1/2 dephosphorylation in hepatocellular carcinoma.

Zhao L, Si K, Luo S, Zhang L, Mao S, Zhang W
Journal of experimental & clinical cancer research : CR 2025 Jul 11;44(1):200

Network pharmacology, molecular docking, and experimental verification to reveal the mechanism of colquhounia root tablet in the treatment of diabetic nephropathy.

Chen D, Wang S, Sun Z, Zhang X, Chong N, Shu J, Wang Q, Xu Y
Biochemical and biophysical research communications 2025 Jan;745:151086

SOX17 Prevents Endothelial-Mesenchymal Transition of Pulmonary Arterial Endothelial Cells in Pulmonary Hypertension through Mediating TGF-β/Smad2/3 Signaling.

Zou X, Yuan M, Zhou W, Cai A, Cheng Y, Zhan Z, Zhang Y, Pan Z, Hu X, Zheng S, Liu T, Huang P
American journal of respiratory cell and molecular biology 2025 Apr;72(4):364-379

Apurinic/apyrimidinic endodeoxyribonuclease 1 (APE1) promotes stress granule formation via YBX1 phosphorylation in ovarian cancer.

Mao S, Xie C, Liu Y, Zhao Y, Li M, Gao H, Xiao Y, Zou Y, Zheng Z, Gao Y, Xie J, Tian B, Wang L, Hua Y, Xu H
Cellular and molecular life sciences : CMLS 2024 Mar 4;81(1):113

Role of ZIP kinase in development of myofibroblast differentiation from HPMCs.

Choo YY, Sakai T, Ikebe R, Jeffers A, Idell S, Tucker TA, Ikebe M
American journal of physiology. Lung cellular and molecular physiology 2024 Mar 1;326(3):L353-L366

Differential expression of PPP1R12A transcripts, including those harbouring alternatively spliced micro-exons, in placentae from complicated pregnancies.

Frew E, Sainty R, Chappell-Maor L, Bone C, Daskeviciute D, Russell S, Buhigas C, Iglesias-Platas I, Lartey J, Monk D
Placenta 2024 Jun;151:1-9

Epithelial-Mesenchymal Transition of Cancer Cells on Micropillar Arrays.

Liu R, Wang H, Ding J
ACS applied bio materials 2024 Jun 17;7(6):3997-4006

Gallbladder dysfunction caused by MYPT1 ablation triggers cholestasis-induced hepatic fibrosis in mice.

Wang Y, Jiang ZH, Zhou YW, Qiu TT, Wang H, Zhu MS, Chen X, Zhang XN
Hepatology communications 2024 Jul 1;8(7)

FDCA Attenuates Neuroinflammation and Brain Injury after Cerebral Ischemic Stroke.

Guan X, Wei D, Liang Z, Xie L, Wang Y, Huang Z, Wu J, Pang T
ACS chemical neuroscience 2023 Oct 18;14(20):3839-3854

Morphological and Functional Colonic Defects Caused by a Mutated Thyroid Hormone Receptor α.

Kim M, Kruhlak M, Hoffmann V, Zerfas P, Bishop K, Doolittle WKL, Edmondson EF, Zhu YJ, Cheng SY
Thyroid : official journal of the American Thyroid Association 2023 Feb;33(2):239-250

Pinocembrin attenuates susceptibility to atrial fibrillation in rats with pulmonary arterial hypertension.

Yi Y, Tianxin Y, Zhangchi L, Cui Z, Weiguo W, Bo Y
European journal of pharmacology 2023 Dec 5;960:176169

MYPT1 inhibits the metastasis of renal clear cell carcinoma via the MAPK8/N-cadherin pathway.

Xie Q, Liu R, Zou Z, Feng Y, Huang Y, Xu G, Sun W, Liang Y, Zhong W
FEBS open bio 2022 Nov;12(11):2083-2095

MYPT1 reduction is a pathogenic factor of erectile dysfunction.

Zhao W, Sun J, Yao LY, Hang D, Li YQ, Chen CP, Zhou YW, Chen X, Tao T, Wei LS, Zheng YY, Ge X, Li CJ, Xin ZC, Pan Y, Wang XZ, He WQ, Zhang XN, Yao B, Zhu MS
Communications biology 2022 Jul 25;5(1):744

Chlorogenic acid improves the intestinal barrier by relieving endoplasmic reticulum stress and inhibiting ROCK/MLCK signaling pathways.

Song L, Wu T, Zhang L, Wan J, Ruan Z
Food & function 2022 Apr 20;13(8):4562-4575

MicroRNA-455-5p Contributes to Cholangiocarcinoma Growth and Mediates Galangin's Anti-Tumor Effects.

Deng X, Zuo M, Pei Z, Xie Y, Yang Z, Zhang Z, Jiang M, Kuang D
Journal of Cancer 2021;12(15):4710-4721

Cyclic GMP-Dependent Regulation of Vascular Tone and Blood Pressure Involves Cysteine-Rich LIM-Only Protein 4 (CRP4).

Längst N, Adler J, Schweigert O, Kleusberg F, Cruz Santos M, Knauer A, Sausbier M, Zeller T, Ruth P, Lukowski R
International journal of molecular sciences 2021 Sep 14;22(18)

Mitotic Acetylation of Microtubules Promotes Centrosomal PLK1 Recruitment and Is Required to Maintain Bipolar Spindle Homeostasis.

Rasamizafy SF, Delsert C, Rabeharivelo G, Cau J, Morin N, van Dijk J
Cells 2021 Jul 22;10(8)

Smad4 regulates the nuclear translocation of Nkx2-5 in cardiac differentiation.

Hu W, Dong A, Karasaki K, Sogabe S, Okamoto D, Saigo M, Ishida M, Yoshizumi M, Kokubo H
Scientific reports 2021 Feb 11;11(1):3588

RhoA/ROCK Pathway Activation is Regulated by AT1 Receptor and Participates in Smooth Muscle Migration and Dedifferentiation via Promoting Actin Cytoskeleton Polymerization.

Qi Y, Liang X, Dai F, Guan H, Sun J, Yao W
International journal of molecular sciences 2020 Jul 29;21(15)

Effects of Dexmedetomidine on the RhoA /ROCK/ Nox4 Signaling Pathway in Renal Fibrosis of Diabetic Rats.

Jihua C, Cai C, Xubin B, Yue Y
Open medicine (Warsaw, Poland) 2019;14:890-898

Nicotine facilitates VSMC dysfunction through a miR-200b/RhoGDIA/cytoskeleton module.

Liang D, Wang Z, Yan Z, Hou S, Xu W, Wang L, Shang M, Qiao Z
Scientific reports 2017 Mar 2;7:43798

Madagascine Induces Vasodilatation via Activation of AMPK.

Chen D, Lv B, Kobayashi S, Xiong Y, Sun P, Lin Y, Genovese S, Epifano F, Hou S, Tang F, Ji Y, Yu D
Frontiers in pharmacology 2016;7:435

MicroRNA-223 Attenuates Hypoxia-induced Vascular Remodeling by Targeting RhoB/MLC2 in Pulmonary Arterial Smooth Muscle Cells.

Zeng Y, Zhang X, Kang K, Chen J, Wu Z, Huang J, Lu W, Chen Y, Zhang J, Wang Z, Zhai Y, Qu J, Ramchandran R, Raj JU, Wang J, Gou D
Scientific reports 2016 Apr 28;6:24900

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 22117-1-AP(MYPT1 antibody) at dilution of 1:3000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human heart using 22117-1-AP(MYPT1 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The MYPT1 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human MYPT1. This antibody recognizes human,mouse,rat antigen. The MYPT1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 22117-1-AP.pdf

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