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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP2-29687 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Brk Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Recognizes human phospho-Brk at tyrosine 342.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Brk [p Tyr342] Antibody [NBP2-29687] - Immunoblot Analysis IGF-1 stimulated lysates from 293T cells transfected with Brk (Lane1) and mutant Brk(y342A) (lane2) were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho Brk(y342) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Brk(y342).
