Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
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Validation data
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- Product number
- MA5-15328 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRK Monoclonal Antibody (2H12B8)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15328 targets PTK6 in indirect ELISA, WB applications and shows reactivity with Human samples. The MA5-15328 immunogen is purified recombinant fragment of human PTK6 expressed in E. Coli. . MA5-15328 detects PTK6 which has a predicted molecular weight of approximately 52kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2H12B8
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PTK6 using PTK6 monoclonal antibody (Product # MA5-15328) in HeLa (1), A549 (2) and MCF-7 (3) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PTK6 using PTK6 monoclonal antibody (Product # MA5-15328) in HeLa (1), A549 (2) and MCF-7 (3) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-BRK Monoclonal Antibody (2H12B8) (Product # MA5-15328) and a 48kDa band corresponding to BRK was observed in all the tested cell models. Modified whole cell lysate (1% SDS) (30ug lysate) of Caco-2 (Lane 1), HT-29 (Lane 2), MCF7 (Lane 3), A549 (Lane 4), PANC-1 (Lane 5), and HepG2 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PTK6 using PTK6 monoclonal antibody (Product # MA5-15328) in HeLa (1), A549 (2) and MCF-7 (3) cell lysate.