MA5-15328

antibody from Invitrogen Antibodies

Targeting: PTK6 BRK

Antibody data

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Validation data

Product number: MA5-15328 - Provider product page
Provider: Invitrogen Antibodies
Product name: BRK Monoclonal Antibody (2H12B8)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15328 targets PTK6 in WB applications and shows reactivity with Human samples. The MA5-15328 immunogen is purified recombinant fragment of human PTK6 expressed in E. Coli.
Antibody clone number: 2H12B8
Concentration: Conc. Not Determined

PTK6 protein structure - MA5-15328 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PTK6 using PTK6 monoclonal antibody (Product # MA5-15328) in HeLa (1), A549 (2) and MCF-7 (3) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PTK6 using PTK6 monoclonal antibody (Product # MA5-15328) in HeLa (1), A549 (2) and MCF-7 (3) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-BRK Monoclonal Antibody (2H12B8) (Product # MA5-15328) and a 48kDa band corresponding to BRK was observed in all the tested cell models. Modified whole cell lysate (1% SDS) (30ug lysate) of Caco-2 (Lane 1), HT-29 (Lane 2), MCF7 (Lane 3), A549 (Lane 4), PANC-1 (Lane 5), and HepG2 (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of PTK6 using PTK6 monoclonal antibody (Product # MA5-15328) in HeLa (1), A549 (2) and MCF-7 (3) cell lysate.