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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- ABIN1504586 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-PTK6 Protein tyrosine Kinase 6 (PTK6) antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunoaffinity Chromatography
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
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Supportive validation
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- antibodies-online (provider)
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- Experimental details
- Dot Blot Analysis (Representative lot data):Non-phosphorylated Brk (Lane 1) and phosphorylated (Lane 2) Brk protein were transferred to PVDF membranes and probed with 2 ?L/ml of anti-phospho-Brk (Tyr342) antibody. Proteins were visualized using a Donkey anti-Rabbit IgG conjugated to HRP and a chemiluminescence detection system.
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- antibodies-online (provider)
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- Experimental details
- Immunoblot Analysis: IGF-1 stimulated lysates from 293T cells transfected with Brk (Lane 1) and mutant Brk(Y342A) (lane2) were resolved by electrophoresis, transferred to PVDF, and probed with anti-phospho Brk(Y342) for 2 hours at room temperature. Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Brk(Y342).
