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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX116570 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116570, RRID:AB_11168451
- Product name
- PRMT7 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Prmt7 Deficiency Causes Reduced Skeletal Muscle Oxidative Metabolism and Age-Related Obesity.
Jeong HJ, Lee HJ, Vuong TA, Choi KS, Choi D, Koo SH, Cho SC, Cho H, Kang JS
Diabetes 2016 Jul;65(7):1868-82
Diabetes 2016 Jul;65(7):1868-82
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: PC-3 7.5% SDS PAGE GTX116570 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PRMT7 antibody detects PRMT7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PRMT7 protein stained by PRMT7 antibody (GTX116570) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PRMT7 antibody immunoprecipitates PRMT7 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of PRMT7 protein by 2 £gg of PRMT7 antibody (GTX116570) 7.5% SDS-PAGE The immunoprecipitated PRMT7 protein was detected by PRMT7 antibody (GTX116570) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Hela xenograft, using PRMT7(GTX116570) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using PRMT7(GTX116570) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using PRMT7(GTX116570) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with NIH3T3 chromatin extract and 5 £gg of either control rabbit IgG or anti-PRMT7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Apex2.
